Custom Structural Characterization of Cloning Enzyme Approaches We Offer

• Primary structure characterization

The primary structure of enzymes is the basis of the biological function of proteins, and the determination of its primary structure can help to study the function of proteins. Mass spectrometry can be used to determine the primary structure of proteins, including molecular weight detection, amino acid composition analysis, peptide sequence analysis, and disulfide bond localization, etc. Creative Enzymes provides mass spectrometry-based primary structure detection services for cloning enzymes.

• Molecular weight determination

There are many methods for molecular weight determination, and the commonly used ones mainly include viscosity, gel permeation chromatography, SDS-PAGE, light scattering, and mass spectrometry. Among them, mass spectrometry mainly includes electrospray ionization (ESI) mass spectrometry and matrix-assisted laser desorption ionization (MALDI) mass spectrometry. Compared with other methods, mass spectrometry identifies protein molecular weight with less sample consumption, and has higher sensitivity, resolution and accuracy, which is beneficial for the analysis of complex mixture samples.

• Isoelectric Point Determination

Creative Enzymes is based on capillary catheter isoelectric focusing (cIEF), which enables accurate determination of isoelectric point of any protein. Compared to traditional plate methods, cIEF has the advantages of short time and high efficiency, high accuracy and low sample usage. The technique is applicable to a wide range of amino acids, peptides, recombinant proteins, enzymes, monoclonal antibodies, etc., and has been widely used in clinical analysis.

Our Capabilities

Creative Enzymes provides high quality structural characterization services to better understand enzyme function and mechanism. Our methods include, but are not limited to:

• X-ray crystallography
• NMR spectroscopy
• Hybrid analysis platform
• Computer-aided structure verification and interpretation

Service Requirements

• The buffer for sample liquid storage does not contain Tris, PBS buffer is recommended.
• Direct provision of cloning enzyme samples: animal and plant tissues/organ or lysates containing decoy proteins and target proteins.

Service Process

Our Features

• Creative Enzymes works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
• Creative Enzymes has a proven, advanced structural characterization of cloning enzyme service technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.

Deliverables

• Screening report
• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in structural characterization. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.