Disulfide bonds are an important form of post-translational modification of proteins and a relatively specific type of modification. Enzyme molecules are linked together by various inter- and intra-strand cysteines, which are essential for the enzyme molecule to maintain the correct high-level structure and sustain the necessary biological activity. Therefore, disulfide bonds have been the focus of research in the biochemical analysis of enzyme drugs. The analysis clarifies the various intra- and interchain disulfide bond pairings of the test article and provides experimental evidence to determine these disulfide bond pairings.
Analysis Approaches We Offer
Creative Enzymes offers a highly sensitive HPLC-MS/MS platform that can analyze disulfide bonds in multiple samples from different sources. In addition, we have developed advanced machine learning strategies based on sequence information. In addition, we offer services related to disulfide artifact validation and disulfide engineering. Our featured services include:
Reference theoretical peptide identification of paired disulfide bonds.
Disulfide peptide identity analysis between reference and donor batches.
Algorithmic analysis based on sequence data.
Disulfide bond analysis via HPLC-MS/MS platform.
Disulfide artifact validation.
Disulfide engineering.
Experimental Instruments
High-resolution mass spectrometer
Ultra high performance liquid chromatography
Our Capabilities
Crystallographic techniques for generating high-resolution three-dimensional structures.
Techniques for combining experimental and algorithmic approaches for the analysis of peptide mixtures.
Algorithmic techniques based on sequence data.
Techniques that combine experimental and algorithmic approaches to analyze digested peptide mixtures.
Typical studies involve multiple analytical techniques such as NMR spectroscopy, X-ray crystallography, and liquid chromatography (LC) coupled with mass spectrometry (MS).
Related Services
DSC analysis
Free hydrophobic content analysis
Circular dichroism analysis
Our Features
Creative Enzymes has established a proven enzyme technology platform for a variety of applications. We'll help you design the right disulfide bond analysis service to aid your scientific research.
Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in disulfide bond analysis service.
Creative Enzymes works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
Service Process
Deliverables
Results raw data
Data analysis
Results analysis
Summary of relevant parameters
Why Choose Us?
Creative Enzymes is a professional disulfide bond analysis service provider supporting a wide range of enzyme services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.
