Overview

Genome editing is a powerful tool for studying gene function and developing novel therapeutic approaches. Zinc finger nucleases (ZFNs) are DNA-binding proteins engineered to produce double-strand breaks at specific genomic locations to enable targeted mutations or insertions. However, due to the complexity of protein engineering and the need for precise targeting, designing and cloning ZFNs can be a challenge. This is where Creative Enzymes come in.

Figure 1. Zinc finger nucleases.

Specific Services We Offer

At Creative Enzymes, we offer high quality genome editing services for cloned enzyme zinc finger nucleases. Our team of experts has extensive experience in the design and cloning of zinc finger nucleases for a variety of applications. We use advanced technology and state-of-the-art equipment to ensure efficient and accurate genome editing of your desired target locus. Our services include:

• ZFN Design: We will work with you to design custom ZFNs targeting your desired genomic motifs. our team of experts will provide customized solutions and advice to ensure optimal results.
• Cloning: We will clone the ZFNs into an appropriate expression vector using high quality enzymes and advanced molecular biology techniques.
• Delivery of ZFN expression plasmids: We will deliver ZFN expression plasmids to you, ready for transfection into your cells of interest.
• Transfection Support: We provide guidance on optimal transfection conditions and protocols to ensure efficient delivery of your ZFN expression plasmid into your cells.
• Knockout/Insertion Screening: We will validate the success of ZFN-mediated genome editing using various methods, such as PCR and sequencing.

Sample Requirements

To ensure successful genome editing, we require high-quality DNA samples containing the target locus for editing. The sample should be of sufficient concentration and purity, with minimal genomic DNA contamination.

Deliverables

We provide a detailed report summarizing the ZFN design, cloning strategy, experimental procedures and results. We will also provide ZFN expression plasmids containing the cloned ZFNs.

Advantages

• Expertise: Our team of experts has many years of experience in molecular biology and genome editing. We have the knowledge and expertise to design and execute a successful ZFN-mediated genome editing strategy for your target locus.
• Advanced technology: We use the latest technology and equipment to ensure efficient and accurate genome editing of your desired target locus. Our approach ensures that you receive high-quality results in a timely manner.
• Customized solutions: We work with you to design a ZFN-mediated genome editing strategy that best suits your needs. Our team of experts provides customized solutions and advice to ensure the best results for your project.

Why Choose Us?

At Creative Enzymes, we are committed to providing high quality cloning enzyme zinc finger nuclease genome editing services at competitive prices. Our team of experts has extensive experience in molecular biology and genome editing. We use advanced technology and state-of-the-art equipment to ensure a reliable and efficient genome editing process. We also offer custom solutions to meet your specific project requirements.