During covalent modifications, the restriction enzymes undergo both inactive and active forms of reciprocal changes. Covalent modifications of enzymes include phosphorylation and dephosphorylation, acetylation and deacetylation, methylation and demethylation, and adenylation and deadenylation, among which phosphorylation modifications are the most common. Because the two structural forms of enzymes are catalyzed by other enzymes, thus covalent structural and conformational changes of molecules occur, and changes in the active form and conformation are realized. Creative Enzymes provides you with a variety of restriction enzyme modification to meet your scientific research needs.

Sepcific Restriction Enzyme Covalent Modification Service Approaches We Offer

Some groups on the peptide chain of a restriction enzyme can be reversibly covalently bound to some chemical group, thus changing the activity of the enzyme, a process called covalent modification of the enzyme. Existing restriction enzymes can be modified by certain structural alterations, so that the resulting functional and catalytic activities allow new catalytic reaction pathways. In addition, modifications can enhance biochemical properties, pharmacokinetics and stability.We offer you with the following modification services:

PEG Modification

Covalent modification with the amphiphilic polymer polyethyleneglycol (PEG) is useful for the preparation of therapeutically activeenzymes with reduced antigenicity and enhanced in viva stability.

Small Molecule Modification

Unglycosylated nuclease RNase A was chemically coupled to D-glucosamine yielding mono- and di-glycosylated enzyme.

Cofactors Modification

The potential for chemical modification as a tool for the covalentattachment of cofactors have shown to improve both the stability andefficiency of enzymes.

Advantages

• Our flexible and communicative team works closely with you to adapt to your analytical requirementse
• We help you shape the methodological part of your manuscripte.
• We back up our in-house servers daily to protect your data.

Service Process

Our Features

• We have accumulated many years of essence in the field of restriction enzyme covalent modification service, helping customers to speed up project development and improve the overall success rate of the project.
• At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.

Deliverables

• Related results raw data
• Data analysis
• Image and result analysis
• Results analysis
• Activity assessment report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional enzyme service provider, supporting a variety of analysis services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. Creative Enzymes has accumulated years of quintessence in the field of restriction enzyme covalent modification services, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.