Molecular cloning involves making multiple copies of a single DNA fragment to make it more accessible for study and manipulation. Most commonly, cloning is achieved by inserting one or more DNA fragments of interest (often referred to as "inserts") into a circular self-replicating DNA called a plasmid vector. When the ligated plasmid/insert is introduced into a bacterium, the circular plasmid/insert replicates, producing hundreds of identical "cloned" copies in each cell. Restriction enzyme cloning, or "restriction cloning," uses DNA restriction enzymes to cut vectors and inserts at specific locations so that they can be easily ligated by DNA ligases to form recombinant DNA. Creative Enzymes provides you with a variety of restriction enzyme cloning service to meet your scientific research needs.
Sepcific Restriction Enzyme Cloning Service Approaches We Offer
Origin of Replication: Replication start allows cloned DNA to replicate independently of the genome.
Selection mlarkers: Selection markers allow selective growth agents to ensure that the cells grown contain vectors. In bacteria, most selection markers are antibiotic resistance genes.
Convenient insertion sites: When cloning with restriction enzymes, the most common entry point for your fragment of interest is the polyclonal site or MCS. MCS is a unique set of restriction enzymes that provide multiple enzyme options to introduce your fragment of interest.
Cloning Selection Marker: A marker that indicates that the vector contains the inserted DNA.
Single or Dual Enzyme Cloning
We can use either single or dual restriction enzyme cloning strategies depending on the importance of the available cloning sites and the correct orientation of the insert.
Advantage of Restriction Enzyme Cloning Service
This method has a wealth of resources, such as vectors and bacterial systems.
It is widely known and studied - this method is taught from high school all the way to university.
There are many restriction endonucleases with different recognition sites and potential overhangs.
There are many software solutions available to help you with design and experimental planning.
Service Process
Our Features
We have accumulated many years of essence in the field of restriction enzyme cloning service, helping customers to speed up project development and improve the overall success rate of the project.
At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.
Creative Enzymes has in-depth cooperation with many biology companies and research institutes, and has extensive experience in providing them with high-quality products and services.
Deliverables
Related results raw data
Data analysis
Image and result analysis
Results analysis
Screening report
Summary of relevant parameters
Why Choose Us?
Creative Enzymes is a company that provides professional and comprehensive restriction enzyme cloning service. We have years of experience to meet your specific project needs. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.
