The study of cloning enzyme's interactions can elucidate the mechanisms of biological reactions and reveal the nature of life phenomena. Although some proteins can function as monomers, most proteins function through interprotein interactions or by forming protein complexes. In recent years, techniques to study molecular interactions have emerged. Creative Enzymes provides protein interaction detection services, including immunoprecipitation (CO-IP, Co-Immunoprecipitation) and GST pull-down, which enable qualitative and quantitative analysis of cloning enzyme and other interactions. Creative Enzymes offers s interaction analysis of cloning enzyme service for the discovery of new enzymes based on years of experience.

Custom Interaction Analysis of Cloning Enzyme Approaches We Offer

Creative Enzymes is a company focused on cloning enzyme research, offering customized services for cloning enzyme interaction analysis with the help of advanced experimental strategies and data analysis tools. Based on different purposes, Creative Enzymes can offer several types of protein interaction methods, including in vivo, in vitro and computer simulation methods.

• Experimental methods

Creative Enzymes offers experimental methods for cloning enzyme interaction analysis, including co-immunoprecipitation (co-IP), pull-down assays, cross-linked protein interaction analysis, tag transfer protein interaction analysis, and Far West blotting analysis.

Affinity determination: Affinity report (quantitative analysis): binding constant (Ka), dissociation constants (Kd) and affinity constants (KD); ranking of affinity strengths.

• Computational methods

Creative Enzymes offers experimental methods for cloning enzyme interaction analysis, including binding free energy (MMGBSA) analysis, hydrogen bond analysis service, secondary structure analysis service and residue interaction network service.

Our Capabilities

• High throughput detection: simultaneous detection and quantitative analysis of intermolecular interactions in the concentration range ng/mL to mg/mL.
• Wide range of applications: tolerates a variety of solution environments and directly detects crude samples.
• Real-time analysis without labeling: time and material saving, real-time monitoring.

Service Requirements

1. The amount of sample protein/antibody provided is greater than 50 ug, small molecules are greater than 300 ug.
2. The buffer for sample liquid storage does not contain Tris, PBS buffer is recommended.
3. Direct provision of cloning enzyme samples: animal and plant tissues/organ or lysates containing decoy proteins and target proteins.

Service Process

Our Features

• Creative Enzymes specializes in enzymatic research and can customize our assay services to meet your assay requirements.
• Creative Enzymes has an extensive database of small molecules to find/screen for interacting small molecules.
• Creative Enzymes can detect in vitro/in vivo, weak/strong, transient/stable interactions.

Deliverables

• Screening report
• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes's cloning enzyme interaction assays are sensitive and measure weak affinity interactions, we can monitor in real time and measure transient interactions; experiments can obtain detailed data for quantitative analysis of cloning enzyme interactions, and ranking of the interaction forces of different enzymes.