Overview

Structural characterization of DNA-modifying enzymes is an important tool for studying these enzymes, which play an important role in regulating gene expression and maintaining genomic stability. The structure of these enzymes is critical to their function, and understanding their structural features can provide valuable insights into their mechanisms of action.

Methods

X-ray crystallography: This method involves obtaining crystals of a protein and using X-rays to determine its atomic structure. This technique has been widely used to determine the structure of many DNA-modified enzymes.

Nuclear magnetic resonance spectroscopy: This method involves studying the interaction between atomic nuclei and a magnetic field. It is particularly useful for determining the structure of smaller proteins and protein complexes in solution.

Electron microscopy: This method involves the use of electrons to capture images of protein complexes. It is particularly useful for studying large protein complexes and those that may be difficult to crystallize.

Small angle X-ray scattering (SAXS): This method involves analyzing the scattering pattern of X-rays passing through the sample. It is useful for determining the overall shape and size of proteins and protein complexes.

Sepecific DNA Modifying Enzymes Structure Characterization We Offer

We offer comprehensive structural characterization services for DNA modifying enzymes, which include the following:

• DNA modifying enzyme production: We produce recombinant DNA modifying enzymes in both prokaryotic and eukaryotic expression systems.
• DNA modifying enzyme purification: We purify recombinant proteins using different chromatographic techniques, including affinity chromatography, ion exchange chromatography, and size exclusion chromatography.
• Structural Analysis: We use various techniques including X-ray crystallography, NMR spectroscopy, electron microscopy, and SAXS to determine the structure of DNA-modified enzymes.
• Structure-based drug design: We use structural information obtained from DNA-modifying enzymes to design small molecule inhibitors that selectively bind these enzymes to modulate their activity.

Applications

• Drug Development: Structural information obtained from DNA-modifying enzymes can be used to design small molecule inhibitors that selectively bind to these enzymes to modulate their activity.
• Epigenetic studies: Structural information obtained from DNA-modifying enzymes can provide a means to understand their mechanism of action and how they interact with other cellular processes.

Our Features

• Customizable Solutions: We offer customizable solutions to meet the unique needs of our customers.
• Fast turnaround times: Our efficient screening and optimization process ensures fast turnaround times for our customers.
• Competitive pricing: We offer competitive pricing without sacrificing quality.

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters
• Structural models of the DNA modifying enzymes
• Electron density maps or NMR spectra
• Detailed protocols for replicating the analysis

Why Choose Us?

Creative Enzymes provides top-notch DNA modifying enzymes structure characterization to assist researchers in exploiting the full potential of these enzymes. Our solutions cover a wide range of applications, from epigenetic research to drug discovery and industrial biotechnology. We have established comprehensive service processes and capabilities to provide personalized services to meet the specific needs of our clients.