DNA modifying enzymes play an important role in the regulation of gene expression and other cellular processes. Understanding the DNA sequences that these enzymes bind to can provide valuable insights into their function and potential therapeutic applications. In this service, we will describe our DNA modifying enzymes motif discovery service, which uses cutting-edge bioinformatics tools to identify DNA motifs that are bound by these enzymes.
Sepecific Motif Discovery Services We Offer
We offer a comprehensive DNA modifying enzyme subject discovery service to help researchers better understand the DNA sequences to which these enzymes bind. Our services include the following steps:
Data pre-processing: We pre-process your DNA sequencing data to remove low-quality reads, trim adapter sequences, and filter out any contaminating sequences.
Peak calling: We use a peak calling algorithm to identify regions of DNA sequences that are bound by DNA modifying enzymes.
Motif Discovery: Using computational methods such as ab initio motif discovery or known motif enrichment analysis, we identify DNA motifs enriched in identified DNA sequence regions.
Motif Validation: We use independent experimental techniques such as electrophoretic mobility shift assays (EMSA) or chromatin immunoprecipitation (ChIP) to validate the identified DNA motifs.
Sample Requirements
Sample type: We accept genomic DNA samples of any biological origin, including animal, plant or microbial samples.
Sample quantity: We require a minimum of 10 µg of high quality genomic DNA for library preparation and sequencing.
Sample Quality: We require high quality genomic DNA samples with a concentration of at least 50 ng/ul and an A260/A280 ratio of 1.8-2.0. This ensures that the DNA is not degraded and is suitable for library preparation and sequencing.
DNA modifying enzyme treatment: We need information about the DNA modifying enzyme treatment, including the type of enzyme used, the concentration and the duration of treatment. This information is necessary to identify the regions of the genome that are bound by DNA modifying enzymes.
Process
Our Features
For each custom project, we assign a team of expert chemists and biologists to help our partners define their research path and goals.
We also provide full assistance in realizing the scientific and business plan, as well as delivering the scientific and business plan.
Applications
Regulatory element discovery: Our services can be used to identify DNA motifs bound by DNA modifying enzymes and potentially discover new regulatory elements that control gene expression.
Disease research: Our services can be used to identify DNA patterns bound by enzymes involved in disease pathways that have the potential to become targets for therapeutic intervention.
Why Choose Us?
Creative Enzymes offers DNA modifying enzymes motif discovery service for DNA modifying enzymes. Our experienced team provides customized solutions based on your specific needs, ensuring high quality and reliable results. We also provide ongoing technical support to ensure the successful implementation of the assay in your study. If you would like to know more about this service, please feel free to contact us.
