Restriction enzymes are so prevalent in the laboratory that it is easy to forget that these enzymes are naturally occurring in bacteria and that their original purpose was not to clone and identify plasmids. Restriction enzymes have been shown to be part of a restriction modification system used by prokaryotes to protect themselves from invasion by exogenous DNA. Another part of this system is methyltransferases, which methylate DNA at specific sequences and protect it from degradation by restriction endonucleases. One or more restriction modification systems are encoded in a particular prokaryotic organism and contain methyltransferases that add methylated groups to specific DNA sequences and nucleic acid endonucleases that recognize specific DNA sequences that are not methylated (e.g., DNA from phages), with both enzymes of the same restriction modification system recognizing the same sites. Creative Enzymes offer a one-stop restriction enzyme methylation service that can meet your research needs for restriction enzyme research.

Sepcific Restriction Enzyme Methylation Service Approaches We Offer

• Controlling Methylation

Creative Enzymes can control methylation by selecting the right strain. To use a restriction site that can be hind  ered by Dam or Dcm methylation, the plasmid can be cloned in advance in an E. coli that does not contain Dam and Dcm methylesterase such as JM110, and then the plasmid can be purified. These particular E. coli strains have been engineered to be Dam and Dcm methylation-deficient strains, producing site-specific unmethylated DNA.

• How to tell if the selected restriction enzyme is available

Before using restriction endonucleases for cloning and identification, it is recommended to perform multiple checks to ensure that the results are not affected by methylation. It is good to know that most restriction enzymes commonly used in laboratories do not have problems with overlapping restriction sites.

Creative Enzymes Commonly used laboratory strains of E. coli K12 such as DH5 contain three methylesterases that recognize and methylate bases of different DNA sequences:

• Dam methylesterase: adds a methylation group to the adenine in the GATC sequence
• Dcm methylesterase: adds a methylation group to the second cytosine of the CCWGG sequence
• EcoKI methylesterase: adds a methylation group to the adenine of AACNNNNNNNGTGC or GCACNNNNNNGTT

Service Process

Our Features

• We have accumulated many years of essence in the field of enzyme service, helping customers to speed up project development and improve the overall success rate of the project.
• At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.

Deliverables

• Results raw data
• Experiment report
• Data analysis
• Image and result analysis
• Results analysis
• Summary of relevant parameters

Advantages

• Our bioinformatics team constantly strives to optimize the pipeline to deliver biologically meaningful results in a short around time
• Our flexible and communicative team works closely with you to adapt to your analytical requirementse
• We help you shape the methodological part of your manuscripte.
• We back up our in-house servers daily to protect your data.
• We have the computing power to take on large projects.

Why Choose Us?

Creative Enzymes is a professional enzyme service provider, supporting a variety of analysis services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. Creative Enzymes has accumulated years of quintessence in the field of restriction enzyme methylation service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.