Restriction enzyme is an enzyme that cuts DNA into fragments at or near a specific recognition site within a molecule called a restriction site. Restriction enzymes are part of a broader class of nucleic acid endonucleases. Restriction endonucleases are usually divided into five types, which differ in their structure and in whether they cut DNA substrates at their recognition sites, or if the recognition site and the cutting site are separate from each other. Creative Enzymes provides you with a variety of restriction enzyme target screening service to meet your scientific research needs.
Creative Enzymes offers a wide range of restriction enzyme target screening services to clients worldwide. Relying on an outstanding team of scientists, we are proud to offer screening services that identify specific target enzymes. Based on expertise, experience and in-house databases, we are able to develop and perform assays specifically designed for target enzymes as the first step in enzyme engineering, ligand design and pathway modulation.
Direct methods involve labeling the restriction enzyme or small molecule of interest, incubating the two populations and directly detecting the binding, usually after some type of washing procedure.
Genetic manipulation can be used to identify restriction enzyme targets by modulating the putative target cell, thereby altering the sensitivity of the small molecule.
Target hypotheses can be generated by computational inference, using pattern recognition methods to compare small molecule effects with those of known reference molecules or genetic perturbations, and by such tests generating mechanistic hypotheses for new compounds, rather than the targets themselves.
At Creative Enzymes, our goal is to apply innovative technologies to restriction enzyme target screening service. We offer innovative, flexible and scalable solutions that can meet the needs of our customers. We strive to meet this challenge as a global company that places a high priority on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality. If you would like to know more about this service, please feel free to contact us.
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