Background

Molecular weight is the basic physicochemical property parameter of an organic compound and generally refers to the relative molecular mass, which is the sum of the relative atomic masses of the individual atoms in the chemical formula of the compound. The correct molecular weight often represents the correct structure of the organic compound or biological macromolecule being measured. Molecular weight is the primary parameter in the identification of enzymes, proteins, etc. It is also one of the important data for the approval of genetically engineered products.

Analysis Approaches We Offer

There are many methods for protein molecular weight determination, Creative Enzymes currently commonly used mainly include:

Mass spectrometry mainly includes electrospray ionization (ESI) mass spectrometry and matrix-assisted laser desorption ionization (MALDI) mass spectrometry. Compared with other methods, mass spectrometry identifies protein molecular weight with less sample consumption, and has higher sensitivity, resolution and accuracy, which is beneficial for the analysis of complex mixture samples. In addition, mass spectrometry can also determine the molecular weights of protein complexes and protein-small molecule complexes.

Our Capabilities

Creative Enzymes offers MALDI-TOF and ESI-MS mass spectrometry platforms for molecular weight determination of various enzyme samples to meet a wide range of protein molecular weight analysis needs, including peptide fingerprinting and protein identification.

• High-resolution mass spectrometry for molecular weight identification

Creative Enzymes mass spectrometry for cloning enzyme identification consists of two main types of mass spectra: one is MALDI-TOF for direct molecular weight measurement, and the other is ESI-MS high resolution mass spectrometry for analysis of multi-charge signals obtained from electrospray, followed by deconvolution analysis of the signals to obtain accurate molecular weight values.

• Precise Mass Determination

Precision mass determination can be used in all areas of chemistry and biochemistry. It can be used to identify unknown compounds in complex mixtures, thereby simplifying the identification of these components. Creative Enzymes offers precision mass determination services for proteins, oligonucleotides and oligosaccharides. We ensure more reliable and accurate results through regular instrument calibration, equipment maintenance, and standard curve development.

Service Advantages

• Advanced technology platforms: we are equipped with a variety of technology platforms to accurately determine purity and homogeneity.
• Accuracy and reproducibility: we provide accurate and detailed results regarding sample purity and homogeneity, thus saving time wasted on bad samples.
• Wide range of applications: purified and homogenized proteins can be used for research and medical applications, such as development and quality control of therapeutic proteins.

Service Process

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes has accumulated years of quintessence in the field of cloning enzyme service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.