Some biological applications may require the introduction of functional groups into the target macromolecule or junction. If no two types of functional groups are available and chemically compatible, the derivatization process of splicing cannot be performed. Knowing which reactive group binds to a target function provides the means to intelligently design modification or affixation strategies. creative Enzymes regularly performs custom functional group modification and derivatization services to alter natural charges, block or expose reactive binding sites, inactivate (and/or activate) functions, modify antibodies, antibody fragments, enzymes, proteins, functional groups of amino acids, peptides, mimetic peptides, nucleic acids, oligonucleotides and functionalized surfaces for biomolecular immobilization.
Procedure
All custom synthesis, modification or biocoupling services for biomolecules are produced under a strict quality control process. We perform analytical HPLC and MS analyses during each development cycle. The final target conjugate must first be isolated from excess or unreacted reagents .
In many cases, a simple dialysis is sufficient to remove unreacted reagents from the reaction solution. Depending on the scope of the project, size exclusion chromatography (SEC) or HPLC can also be used to remove excess reagents or to isolate and characterize cross-linked products. The cross-linked target molecule can then be further characterized by biochemical or biophysical techniques.
Once a product has been purified, it may be subject to a variety of characterization methods including spectroscopy (MALDI-TOF, ESI, LC-MS), fluorescence, electrophoresis, immunochemistry, biochemical and enzymatic assays. QC (quality control) and QA (quality assurance) procedures are also followed independently to ensure the highest quality of each coupling delivered. In addition, our dedicated technical account manager will guide you through each step of the process and keep you up to date on the progress of your project.
Our Capabilities
Excellent technical support: Assisting customers from design to delivery.
High success rate: Creative Enzymes custom biocouples have a success rate of over 95%, higher than our competitors.
Full range of modification and activation services: modification and derivatization of small molecules, biopolymers, small molecules, and creation of functionalized surfaces for immobilization and cross-linking.
Flexible technologies: synthesis and modification of functional target molecules using solid/liquid phase and microwave techniques.
Optimized chemistry: expertise in hundreds of crosslinking modification, activation and/or affixation techniques using reactive crosslinkers.
Quality assurance: all samples are carefully monitored for stability and characterized to ensure batch-to-batch consistency.
Service Process
Our Features
Creative Enzymes has established a proven enzyme technology platform for a variety of applications. We'll help you design the right biomolecule modification to aid your scientific research.
Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in biomolecule modification technology service.
Deliverables
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Data analysis
Results analysis
Summary of relevant parameters
Why Choose Us?
Creative Enzymes is a professional biomolecule modification provider supporting a wide range of enzyme services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.
