DNA modifying enzymes are a class of biologically active proteins that have been used in a wide variety of applications such as gene therapy, molecular biology and biotechnology. purification of DNA modifying enzymes is essential to ensure their optimal activity and stability. Creative Enzymes offers a comprehensive DNA modifying enzyme purification service that can handle even the most challenging samples.
Overall Solutions
Creative Enzymes offers comprehensive DNA modifying enzyme purification services from start to finish.
We begin with a consultation to determine your specific needs and develop an appropriate purification strategy.
Our team then performs the purification using the most appropriate method and analyzes the resulting product using a variety of techniques such as SDS-PAGE, HPLC and mass spectrometry.
We can also perform activity assays to confirm the functionality of the purified enzyme.
Sepecific DNA Modifying Enzymes Purification We Offer
Creative Enzymes utilizes specialized techniques and methods to purify DNA modifying enzymes. Our team of experts has extensive experience in the field of DNA modifying enzyme purification and uses a variety of techniques to achieve the highest levels of purity. Some of the methods we use include:
Affinity chromatography
Ion exchange chromatography
Size exclusion chromatography
Hydrophobic interaction chromatography
Service Process
Consultation: We discuss your purification needs and then develop a purification strategy.
Sample preparation and purification: We guide you through the best methods to prepare your purified samples.
Analysis: We analyze the purified product using various techniques such as SDS-PAGE, HPLC and mass spectrometry.
Activity assay: We perform activity assays to confirm the functionality of the purified enzyme.
Sample Requirements
We need a sufficient amount of sample material (typically 10-100 mg). Samples should be of high quality and free of contaminants, e.g., nucleic acids, lipids or salts. If needed, we can provide guidance on the best way to prepare the sample.
Our Capabilities
Creative Enzymes can purify various types of DNA modifying enzymes, including but not limited to:
Restriction Enzymes: enzymes that cleave DNA at a specific nucleotide sequence.
DNA methyltransferases: enzymes that transfer a methyl group to DNA.
DNA ligases: enzymes that join two DNA fragments together.
Polymerase: Enzyme that synthesizes DNA.
Topoisomerases: Enzymes that change the topology of DNA.
Our Features
Expertise: Our team has extensive experience in protein purification and uses state-of-the-art technology.
Customization: We develop customized solutions to meet your specific needs.
Quality: We put quality at the forefront of every step of the purification process to ensure high purity products.
Deliverables
Results raw data
Data analysis
Results analysis
Summary of relevant parameters
Why Choose Us?
At Ceative Enzymes, our goal is to apply innovative technologies to end.We offer innovative, flexible and scalable solutions that can meet the needs of our customers. We strive to meet this challenge as a global company that places a high priority on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality. If you would like to know more about this service, please feel free to contact us.
