Homologous recombination is a process by which DNA sequences with similar or identical regions can be exchanged for genetic material. In molecular biology, homologous recombination is widely used in cloning applications to insert DNA fragments into plasmids or other vectors. This process involves the use of various enzymes, including restriction endonucleases, DNA ligases, polymerases and recombinases. Creative Enzymes is the premier provider of cloning enzyme homologous recombination services for researchers in the biotechnology industry. Our team of experts has extensive experience in the production, purification and characterization of enzymes, including those critical for homologous recombination-based cloning.
Specific Services We Offer
At Creative Enzymes, we offer a comprehensive package of homologous recombination services, including the production, purification and characterization of the enzymes involved in the cloning. Our team uses advanced techniques to optimize enzyme expression in bacterial or yeast systems, ensuring high yields of active enzymes. Purified enzymes are characterized by SDS-PAGE and activity assays to confirm their purity and function.
Workflow
Production: We use proprietary protocols to produce enzymes involved in homologous recombination in bacterial or yeast systems. Customers can choose from several expression host and vector options, depending on their specific needs.
Purification: After producing the enzyme, our team performs a series of purification steps to separate it from other proteins and contaminants. We utilize various chromatographic methods such as ion exchange, affinity and size exclusion chromatography to achieve high purity levels.
Characterization: We use SDS-PAGE and activity assays to assess the purity and functionality of the purified enzymes. Our team ensures that these enzymes are free of nucleases, proteases and other impurities that could interfere with downstream applications.
Cloning: We offer cloning services based on homologous recombination, where DNA fragments are inserted into plasmids or other vectors. This process involves the use of various enzymes such as restriction endonucleases, DNA ligases, polymerases and recombinases to ensure efficient and accurate insertion of DNA fragments.
Sample Requirements
In order to perform homologous recombination services, we require high quality DNA samples, including plasmid DNA or genomic DNA, at a concentration of at least 50 ng/µl.
DNA should be pure and free of contaminants that could interfere with enzyme activity or the homologous recombination process.
Customers can submit their samples in a variety of formats including lyophilized powders, frozen pellets, or liquid solutions, and we recommend that customers consult with our team prior to submitting samples to ensure proper handling and storage.
Deliverables
At Creative Enzymes, we provide our customers with comprehensive results reports detailing the production, purification, characterization and cloning of enzymes involved in homologous recombination. The reports include information on the yield, purity and activity of the enzyme, as well as any technical details related to the production process.
Our Features
High Quality Enzymes: Our team employs stringent quality control measures throughout the production and purification process to ensure our customers receive enzymes of the highest purity and activity.
Customized service: We understand that each project has unique requirements, and we work closely with our customers to tailor our services to their specific needs. Our team provides expert advice and support throughout every step of the process.
Why Choose Us?
Creative Enzymes is a leading provider of homologous recombination-based cloning services to researchers in the biotechnology industry. With our expertise in enzyme production, purification and characterization, we can provide high quality enzymes involved in homologous recombination for a variety of molecular biology applications. Our team is committed to providing customized services to meet the unique needs of each project, and we pride ourselves on delivering results in a timely and cost-effective manner. Contact us today to learn more about our homologous recombination services and how we can support your research.
