Overview

Cloning and expression of recombinant proteins is a key step in many research projects. However, it can be a time-consuming and challenging process that requires a great deal of expertise. One of the most important steps in this process is to verify that the expressed proteins are of the expected quality and quantity. This is where our cloning enzyme protein expression validation services come in. We offer a comprehensive service to help researchers validate their protein expression, saving them time and ensuring the success of their projects.

Specific Services We Offer

• Our cloning enzyme protein expression validation services cover a range of projects, from small-scale expression optimization to large-scale recombinant protein production.
• We work with a variety of organisms, including bacteria, yeast, insect cells and mammalian cells.
• Our team of experts has experience in all aspects of protein expression, from vector design and cloning to protein purification and characterization.

Our Methodology

Our services use a range of methods to validate protein expression, including SDS-PAGE, Western blot, ELISA, activity assays, and mass spectrometry.

We also use advanced techniques such as thermal transfer assays, which can provide information on protein stability and ligand binding. Our team is experienced in solving expression problems and optimizing protein production to improve yield and quality.

Sample Requirements

• We need the DNA sequence of the gene of interest and the desired expression system.
• We can build the vector in-house using a customer-supplied plasmid or using our extensive cloning enzyme library.
• We also need information on the desired protein purification method and any specific requirements for downstream applications.

Deliverables

We provide our customers with a comprehensive report detailing the results of their protein expression validation. This report includes data on protein expression levels, purity and activity, as well as any problems encountered during the expression process.

Applications

• Our cloning enzyme protein expression validation service is ideal for researchers who need to express recombinant proteins for a variety of applications, including structural biology, drug discovery, and biotechnology.
• Our services can be tailored to the specific needs of each project, whether it involves small-scale expression for screening purposes or large-scale pure protein production for structural studies.

Why Choose Us?

At Creative Enzymes, our goal is to apply innovative technologies to cloning enzyme expression validation service. We offer innovative, flexible and scalable solutions that can meet the needs of our customers. We strive to meet this challenge as a global company that places a high priority on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality. If you would like to know more about this service, please feel free to contact us.