DNA modifying enzymes play a key role in regulating gene expression and maintaining genomic stability. Understanding the activity of these enzymes is critical to understanding their role in gene regulation and disease. Spectrophotometric assays are widely used to measure enzyme activity due to their high sensitivity and accuracy. These assays rely on measuring the change in absorbance at a specific wavelength due to an enzyme reaction.
Methods
Our DNA modifying enzyme spectrophotometric assay service is based on the measurement of the change in absorbance at a specific wavelength produced by the enzymatic reaction.
The spectrophotometric assay service typically involves incubating a sample containing the enzyme of interest with a substrate, which is then converted by the enzyme into a product. A spectrophotometer is used to measure the change in absorbance resulting from the conversion of the substrate to the product.
The specific method used for each assay may vary depending on the enzyme and substrate being studied. For example, DNA methyltransferase assays typically involve the use of radiolabeled S-adenosylmethionine (SAM) as a methyl donor, while DNA glycosylase assays typically involve the use of DNA substrates containing modified bases.
Service We Offer
We offer a spectrophotometric assay service for DNA modifying enzymes that accurately measures the activity of enzymes involved in DNA modification, including DNA methyltransferases, DNA demethylases and DNA glycosylases. Our services provide researchers with a reliable method of measuring enzyme activity, which is critical to understanding the role of these enzymes in gene regulation and disease.
Sample requirements
We require a minimum of 1 μg of purified enzyme or cell lysate per assay. We recommend that samples be frozen at -80°C until shipment.
Applications
Our DNA modifying enzyme spectrophotometric assay service can be used for a range of applications, including:
Measuring DNA modifying enzyme activity in vitro
Determining the substrate specificity and kinetic profile of DNA modifying enzymes
Screening compounds for DNA modifying enzyme activity
Investigating the role of DNA modifying enzymes in gene regulation and disease
Our Features
Our dedicated team includes senior scientists with expertise in molecular biology, biochemistry and biotechnology.
We use validated and optimized protocols to ensure accurate and reproducible results.
We are committed to providing high quality services to meet the needs of our clients and exceed their expectations.
Deliverables
Results raw data
Data analysis
Results analysis
Summary of relevant parameters
Why Choose Us?
Creative Enzymes provides you with a variety of DNA modifying enzymes spectrophotometric assay to meet your scientific research needs. Our experienced team provides customized solutions based on your specific needs, ensuring high quality and reliable results. We also provide ongoing technical support to ensure the successful implementation of the assay in your study. If you would like to know more about this service, please feel free to contact us.
