Some biological applications may require the introduction of functional groups into the target macromolecule or junction. If no two types of functional groups are available and chemically compatible, the derivatization process of splicing cannot be performed. Knowing which reactive group binds to a target function provides the means to intelligently design modification or splicing strategies.Creative Enzymes regularly performs custom functional group modification and derivatization services to alter natural charges, block or expose reactive binding sites, inactivate (and/or activate) functions, modify DNA-modifying enzymes and functionalize surfaces for biomolecular immobilization.
Our Capabilities
Bio-Synthesis can help customers who want to introduce functional groups or modify existing biopolymers, such as natural proteins, oligomers, peptides or commercially available synthetic compounds, to be ready for binding to other biopolymers.
Chemistry of the Reactive Groups Used
Every chemical modification or conjugation process involves the reaction of one functional group with another, resulting in the formation of covalent bonds. We have delivered thousands of custom coupled biopolymers and are well positioned to meet the growing demand for biocoupling in biological and drug discovery research. Our organic laboratory has applied hundreds of reaction systems.
Amine reactions: NHS esters, imine esters, hydroxymethylphosphines, guanidination of amines, fluorophenyl esters, carbodiimides, anhydrides, aromatizers, carbonates, aldehydes and glyoxal
Aldehyde and ketone reactions: hydrazine derivatives, Schiff base formation
Sample Requirements
User-supplied non-commercial small or large molecules should be sufficiently pure (≥95% pure). Please provide QC data (typically HPLC, MS, ESI or NMR data, etc.) and MSDS (if available) along with your compound.
We can assist you in purifying and collecting analytical data. These compounds must contain functional groups that can be targets for crosslinking, including carboxylate groups, primary amine groups, aldehyde/ketone residues, hydroxyl groups, hydrazines, hydrazides, amino oxygens, sugar/polysaccharide groups, or thiol-reactive functional groups.
If necessary, we can also assist you in creating and activating specific functions. Any samples provided by the customer will require quality checks by our analytical team to ensure sample quality prior to coupling.
Service Process
Our Features
Creative Enzymes has established a proven enzyme technology platform for a variety of applications. We'll help you design the right DNA modifying enzyme modification service to aid your scientific research.
Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in DNA modifying enzyme modification service.
Deliverables
Results raw data
Data analysis
Results analysis
Summary of relevant parameters
Why Choose Us?
Creative Enzymes is a professional DNA modifying enzyme modification service provider supporting a wide range of enzyme services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. WIf you would like to know more about this service, please feel free to contact us.
