DNA demethylation, which is defined as the lack and reduction of intracellular DNA methyltransferase content in mammalian cells that causes DNA demethylation, is important for turning on the initiation of specific gene expression and reprogramming. The mechanism of active DNA demethylation has long been one of the most fundamental questions in the field of epigenetics. In contrast to passive demethylation, active DNA demethylation is a rapid and cell division-independent process.
Creative Enzymes provides high quality DNA demethylase assays to researchers around the world. Our experienced team of professionals can assist with all aspects of the assay, from experimental design to interpretation of results. Our services include:
Assay design: Our experts can help select the appropriate substrate, enzyme source and assay for each project.
Assay optimization: We optimize assay conditions to ensure accurate and reproducible results. We test various parameters such as enzyme concentration, substrate concentration, pH and incubation time to achieve optimal assay performance.
Assay validation: We validate each assay to ensure its accuracy and reliability. We use positive and negative controls to determine the sensitivity, specificity and reproducibility of the assay.
Data Analysis: We provide comprehensive data analysis and interpretation services. Our experts can help interpret results and provide insight into their biological significance.
We require a minimum of 1 μg of purified enzyme or cell lysate per assay. We recommend that samples be frozen at -80°C until shipment.
Sample Preparation: We purify enzyme samples from cell lysates or other sources and measure their protein concentration.
Substrate preparation: We prepare substrates by synthesizing DNA sequences containing methylated CpG dinucleotides or by using commercially available substrates.
Incubation: We incubate the enzyme samples with the substrate under optimized conditions to cause demethylation.
Detection: We detect changes in DNA methylation using various techniques, such as PCR, bisulfite sequencing, or methylation-specific qPCR.
Data interpretation: We analyze and interpret the data obtained from the assays to draw meaningful conclusions.
Our assays can be used to screen for potential inhibitors or activators of DNA demethylases for drug discovery.
Our assays can be used to study the role of DNA demethylases in epigenetic regulation and gene expression.
Aberrant DNA methylation patterns are frequently observed in cancer, and our assays can be used to elucidate the mechanisms behind these changes and to identify potential therapeutic targets.
We provide our clients with detailed reports that include all experimental procedures and results obtained. The report includes a summary of the assay design, optimization, validation and data analysis.
Creative Enzymes offers DNA demethylase assay service for DNA modifying enzymes. Our experienced team provides customized solutions based on your specific needs, ensuring high quality and reliable results. We also provide ongoing technical support to ensure the successful implementation of the assay in your study. If you would like to know more about this service, please feel free to contact us.
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