Cloning enzymeis an important tool in the field of molecular biology that facilitates the amplification and cloning of DNA fragments. Plasmid purification is an important process in molecular cloning, which involves the isolation of plasmid DNA from bacterial cells. purified plasmids can be used for various downstream applications such as sequencing, gene expression analysis and transfection. Cloning enzymeplasmid purification services provide researchers with a convenient and efficient way to obtain high-quality plasmid DNA for their experiments.
Sepecific Services We Offer
Our services include bacterial culture, lysis, DNA binding, washing and elution steps to ensure maximum yield and purity of plasmid DNA.
Our cloning enzyme plasmid purification service is based on alkaline lysis, a widely used and reliable method of plasmid purification.
Bacterial cells are first harvested and lysed using a solution containing sodium hydroxide and sodium dodecyl sulfate (SDS). This lysis solution denatures the proteins and lyses the cell membrane, releasing the DNA into the solution.
The mixture is then neutralized with potassium acetate and the resulting precipitate contains genomic DNA, RNA and proteins. Plasmid DNA is selectively bound to the silica-based resin column, while other contaminants are washed away.
Finally, the plasmid DNA is eluted from the column and can be further purified if desired.
We also offer custom services to meet specific customer requirements.
Sample Requirements
Customers are required to provide bacterial cultures containing the desired plasmids.
Bacterial cultures can be provided in liquid or solid form, depending on the customer's preference.
We recommend providing a minimum of 1-2 ml of bacterial culture for best results.
Customers should also provide information on plasmid type, size, copy number and antibiotic resistance (if applicable).
Deliverables
Our cloning enzyme plasmid purification service provides high quality plasmid DNA that meets or exceeds our customers' expectations.
Purified DNA is provided in a suitable buffer, and its concentration and purity levels are determined spectrophotometrically.
We also provide a detailed report with information on the purification process, yield, purity and concentration of plasmid DNA.
Our Features
We utilize state-of-the-art equipment and protocols to ensure the highest quality of purified DNA.
Our services are customizable to meet the specific requirements of our clients, making them ideal for academic and industrial research.
Finally, our pricing is competitive, and we offer flexible payment options to accommodate different budgets.
Applications
Our service is suitable for various downstream applications such as sequencing, gene expression analysis and transfection.
It can be used for molecular cloning experiments, recombinant protein expression and gene therapy studies.
The high quality plasmid DNA obtained from our cloning enzymeplasmid purification service ensures accurate results and reliable performance for these applications.
Why Choose Us?
At Creative Enzymes, our goal is to apply innovative technologies to custom cloning enzyme plasmid purification service. We offer innovative, flexible and scalable solutions that can meet the needs of our customers. We strive to meet this challenge as a global company that places a high priority on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality. If you would like to know more about this service, please feel free to contact us.
