Chemical modification of restriction enzymes is a technique to modify enzyme molecules by chemical methods, such as cutting and splicing of the main chain and chemical modification of the side chain groups, in order to change the physicochemical properties and biological activity of the restriction enzyme protein. The purpose is to artificially change some properties of natural enzymes, create some excellent properties or even create new activities that natural enzymes do not have, so as to expand the application of enzymes and promote the development of biotechnology. Creative Enzymes provides you with a variety of restriction enzyme modification to meet your scientific research needs.

Sepcific Restriction Enzyme Chemical Modification Service Approaches We Offer

• Site-specific Mutation

Creative Enzymes has started to introduce specific molecules for modification at specific sites of enzymes or proteins through a number of controlled methods, combined with fixed-point mutations to introduce a non-natural amino acid side chain for chemical modification, resulting in a number of novel enzyme formulations. This strategy involves the introduction of an amino acid residue at the key active site of the enzyme using fixed-point mutagenesis, followed by the modification of the mutated amino acid residue using chemical modification to introduce a small molecule compound to obtain a chemically modified mutant enzyme.

• Cross-linking Technology

Creative Enzymes use bifunctional group reagents such as glutaraldehyde and PEG to covalently cross-link the enzyme protein molecules between each other, between subunits or different peptide chain parts within the molecule, which can reinforce the active structure of the molecule and can improve its stability, expanding the use of the enzyme in non-aqueous solvents.

• Small molecule compounds

Chemical modification of the enzyme active site or side chain groups outside the active site using small molecule compounds can change the enzymatic properties.

Advantages

•  Our chemical modifications can enhance the biological activity of the restriction enzymes, including some changes in the reaction properties of the effector after modification.
• Enhanced stability in adverse environments (non-physiological conditions).
• Reduced biorecognition in response to allogeneic reactions.

Service Process

Our Features

• We have accumulated many years of essence in the field of restriction enzyme chemical modification services, helping customers to speed up project development and improve the overall success rate of the project.
• At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.

Deliverables

• Related results raw data
• Data analysis
• Image and result analysis
• Results analysis
• Activity assessment report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional restriction enzyme chemical modification service provider, supporting a variety of enzyme-related services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. Creative Enzymes has accumulated years of quintessence in the field of restriction enzyme chemical modification services, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.