Each restriction enzyme has optimal reaction (assay) conditions and different conditions for long term storage. The recommended assay and storage conditions are determined by the manufacturer in order to provide the user with the highest activity, best fidelity and maximum stability for each enzyme. Factors that must be considered include temperature, pH, enzyme cofactor, salt composition, ionic strength and stabilizer. Setting up a digestion using a single restriction enzyme is relatively simple. However, digestions using multiple enzymes with different buffer requirements may require the use of alternative buffers and may require adjustment of the number of units of enzyme used. Creative Enzymes offer a one-stop standard restriction enzyme reaction service that can meet your research needs for restriction enzyme research.
| Reaction Conditions Control | Description |
| pH | Most restriction enzymes are used between pH 7.2 and pH 8.5 as measured at the temperature of incubation. |
| Salt Concentration | Restriction enzymes are diverse in their response to ionic strength. Most are stimulated by 50-150 mM NaCl or KCl while others are inhibited by salt concentrations higher than 20 mM. |
| BSA | Bovine Serum Albumin is used in restriction enzyme storage buffers and is added to digestion reactions to stabilize the enzyme. BSA can protect restriction enzymes from proteases, non- specific adsorption and harmful environmental factors such as heat, surface tension and interfering substances. |
| Volume | Viscous DNA solutions inhibit enzyme diffusion and can reduce enzyme activity. DNA concentrations that are too dilute can fall below the Km of the restriction enzyme and also affect enzyme activity. |
| Glycerol | Glycerol is added to restriction enzyme storage buffers to prevent freezing at -20°C. Repeated freeze/thawing of restriction enzymes can reduce their activity. |
Creative Enzymes is a professional enzyme service provider, supporting a variety of restriction enzyme services. We have years of experience to meet your specific project needs. Creative Enzymes has accumulated years of quintessence in the field of standard restriction enzyme reaction service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.
We are here to answer any question you may have
