At Creative Enzymes, we have a wide range of cloning enzyme enzyme stability testing capabilities to support your product development and formulation needs. In addition to our numerous analytical and stability testing services, we have a large team of scientists and laboratory professionals to provide you with personalized service and attention.

Factors Affecting Enzyme Stability

• pH and buffers. Most enzymes are stable only within a specific pH range, beyond which stability is reduced.
• Enzyme concentration. Although enzyme stability varies by nature and purity, in general, enzyme proteins are stable at high concentrations and are prone to dissociation and adsorption at low concentrations, and even to surface denaturation.
• Some enzymes are sulfhydryl enzymes, which will be gradually inactivated by sulfhydryl oxidation in air. In this case, the addition of 1 mmol/L EDFA or DTT helps to improve their stability.

Custom Cloning Enzyme Stability Evaluation Approaches We Offer

• Computational Methods

Creative Enzymes offers a professional cloning enzyme stability analysis service that can help you understand the stability of cloning enzyme and its binding to small molecule drugs or ligands. Our methods include RMSD calculations and RMSF calculations.

Figure 1. RMSD&RMSF. (Sakkiah S, et al.2018)

• We also offer the following services to improve the stability of cloning enzymes, We can add substrates, inhibitors and coenzymes. This is a widely used method:
• Addition of L-glutamate often stabilizes N-methylglutamate synthase
• Addition of citric acid stabilizes aconitase
• Addition of competitive inhibitors such as sodium benzoate or coenzyme FAD
• The addition of SH-protectants such as glutathione, dimercaptoethanol (but prone to auto-oxidation) and DDT.
• Other additives. Certain low molecular inorganic ions, such as Ca²⁺ to protect α-amylase, Mn²⁺ to stabilize lysozyme, CI^- to stabilize hyaluronidase, etc.

Service Process

Advantages

• With multiple subsets of libraries designed for specific screening needs.
• Each candidate is up-to-date, less than 2 years old at the time of selection.
• Candidates are rigorously screened to exclude undesirable features.
• All candidate compounds or building blocks have test specifications.

Our Features

• 24-hour continuous monitoring: ensure that your test results are not only dependent on your protocol, but also safe, reliable and accurate.
• Real-time acceleration conditions: You have complete flexibility to determine your test conditions at Creative Enzymes.
• A range of custom services: to meet the needs of any project, we can develop and deliver a complete study to meet your customer's needs.

Guaranteed delivery: Creative Enzymes guarantees to help you deliver your project!

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Purification assessment report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a company that provides professional and comprehensive cloning enzyme stability evaluation service. We have years of experience to meet your specific project needs in using enzyme research to add value to your research projects. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.