One of the approaches for cloning enzyme screening is enzyme discovery based on protein 3D structure, since sequence-based approaches usually find enzymes belonging to known families. In addition, the primary structure, amino acid sequence, does not always correlate with some 3D structure, while the function of an enzyme is largely determined by its 3D structure. Therefore, searching by sequence only usually leads to unwanted activity or missing target enzymes. Creative Enzymes offers 3D structure-based cloning enzyme screening strategies for the discovery of new enzymes based on years of experience.

Custom 3D Structure-Based Screening Approaches We Offer

• Creative Enzymes uses an overall 3D structure-based approach for cloning enzymes belonging to families or subfamilies in which at least one member has a resolved structure.
• Creative Enzymes discovers cloning enzymes by focusing on highly conserved regions associated with catalysis, including catalytic residues, cofactors, and substrate binding residues and their relative spatial location to form constellations of minimal catalytic active sites.
• With years of experience in combining different enzyme discovery techniques, we provide the following professional screening services:

Our Capabilities

• Creative Enzymes has put a lot of effort into developing bioinformatics tools to predict the function of 3D structures of proteins, which constitute an extended resource for new enzyme discovery.
• In addition, we have built a database dedicated to catalytic residue annotation for enzymes. Creative Enzymes has extensive experience in providing 3D structure-guided strategies for cloning enzyme discovery.
• We help develop custom assignment strategies for proteins with unknown functions in order to better understand metabolism and enzymology from a fundamental perspective.

Service Process

Our Features

• We have accumulated many years of essence in the field of restriction enzyme covalent modification service, helping customers to speed up project development and improve the overall success rate of the project.
• At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.
• Our flexible and communicative team works closely with you to adapt to your analytical requirementse
• We help you shape the methodological part of your manuscripte.
• We back up our in-house servers daily to protect your data.

Deliverables

• Screening report
• Results raw data
• Data analysis
• Results analysis
• Characterization assessment report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional enzyme service provider, supporting a variety of services. Creative Enzymes has accumulated years of quintessence in the field of 3D structure-based cloning enzyme screening services, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.