RNA-Seq analysis has revolutionized the study of gene expression and is widely used in biomedical, agricultural and industrial applications. DNA modifying enzymes are important tools for manipulating genetic information and understanding their impact on gene expression can provide valuable insights into the role of these enzymes in regulating gene expression. Creative Enzymes provides you with a variety of DNA modifying enzymes RNA-seq analysis service to meet your scientific research needs.
Sepecific Assay Service We Offer
Library Preparation: We use library preparation methods such as poly(A) selection or ribosomal RNA depletion to prepare high quality RNA libraries from your biological samples. This step ensures that the RNA libraries are of high quality and suitable for sequencing.
Sequencing: We perform high-throughput sequencing of the prepared RNA libraries using Illumina or other sequencing platforms. This step generates millions of reads representing the transcriptome of your biological sample.
Differential Expression Analysis: Based on RNA-Seq data, we perform differential expression analysis to identify differentially expressed genes between samples under different experimental conditions or treated with different DNA modifying enzymes.
Functional analysis: We perform functional analysis of the identified differentially expressed genes to determine the functional class or pathway of enrichment. This step can provide valuable insights into biological processes affected by DNA modifying enzymes.
Applications
Gene expression studies: Our services can be used to identify genes that are differentially expressed under different experimental conditions or between samples treated with different DNA modifying enzymes and to study their expression patterns.
Functional studies: Our services can be used to study the effects of DNA-modifying enzymes on specific biological processes and to identify novel roles of these enzymes in gene regulation.
Deliverables
To ensure the success of our DNA modifying enzyme RNA-Seq analysis services, we have the following sample requirements:
Sample type: We accept total RNA or mRNA samples of any biological origin, including animal, plant or microbial samples.
Sample quantity: We require a minimum of 1 µg of high quality RNA for library preparation and sequencing. However, if possible, we recommend submitting larger amounts of RNA to increase the depth of sequencing and improve the accuracy of our analysis.
Sample Quality: We require high quality RNA samples with a RNA Integrity Number (RIN) of at least 7.0, which ensures that the RNA is not degraded and is suitable for library preparation and sequencing.
DNA modifying enzyme treatment: We need information about DNA modifying enzyme treatment, including the type of enzyme used, concentration and treatment time. This information is necessary to perform accurate differential expression analysis and functional analysis.
Why Choose Us?
At Creative Enzymes, our goal is to apply innovative technologies to DNA modifying enzymes RNA-seq analysis. We offer innovative, flexible and scalable solutions that can meet the needs of our customers. We strive to meet this challenge as a global company that places a high priority on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality. If you would like to know more about this service, please feel free to contact us.
