Enzyme-linked immunosorbent assay (ELISA) is used for the determination of serum proteins. but it is more cumbersome It has the disadvantage of not being able to be used for large scale on-line analysis. The use of a homogeneous enzyme immunoassay technique can compensate for this. The homogeneous enzyme immunoassay technique can compensate for the the shortcomings of the method. The method can be used to compensate for the shortcomings of the method. Creative Enzymes services, including method development, validation, transfer and method development, help you accelerate your drug development process based on your every need!
Enzyme immunoassay techniques include two major categories: non-homogeneous enzyme immunoassay techniques and homogeneous enzyme immunoassay techniques. The former is sensitive and inexpensive but has a high error rate due to cumbersome operation. The latter can compensate for the former. However, the homogeneous enzyme immunoassay technique also has disadvantages such as unstable source of β-galactosidase and batch instability, which cannot be used for complete antigen detection. We constructed and modified the prokaryotic expression system of β-galactosidase by recombinant gene technology to divide β-galactosidase into EA and ED subunits, and refined the induction conditions and purification methods for the inductants. The system has been improved and successfully obtained β-galactosidase in large quantities to avoid the disadvantage of unstable source and batch instability. The disadvantages of unstable source and batch instability were avoided.
Creative Enzymes is a professional cloning enzyme homogeneous technology service provider supporting a wide range of enzyme services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years of quintessence in the field of cloning enzyme homogeneous technology service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.
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