Analysis based on primary sequences of whole proteins or specific parts is a common approach to discover cloning enzymes. New enzymes performing the same or similar reactions can be screened and identified using known and characterized enzymes and their corresponding genes in databases. This process can be accomplished by amplifying an unsequenced target gene with designed primers or with the help of a protein sequence comparison tool after the target gene has been sequenced. Creative Enzymes offers sequence-based strategies for the discovery of new enzymes based on years of experience.
Custom Sequence-based Cloning Enzyme Screening Approaches We Offer
Creative Enzymes offers a complete suite of sequence-based screening services. Our service process is as follows:
Step 1: We can propose different sequence-based screening strategies depending on the availability of genomic data.
Step 2: PCR primer sequence-based probe technology can explore complex DNA mixtures of metagenomes for which genomic data are not available.
Step 3: Using this technique, PCR primers are designed using known parental genes as templates and used for PCR amplification of close homologous genes.
Step 4: By combining probe technology with genomic background analysis, new cloning enzymes were identified in strains showing the desired activity.
Step 5: Paired sequence alignment strategy through available genomic data
Step 6: Heterologous expression was used to identify the target novel genes.
Our Capabilities
PCR primer design
Sequence alignment
Heterologous expression strategy design
Candidate cloning enzyme screening
Cloning enzyme expression, purification and characterization
One-stop after-sales service
Service Process
Our Features
Creative Enzymes will complete your project on time and efficiently.
Creative Enzymes works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
Creative Enzymes has a proven, advanced technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.
Deliverables
Screening report
Results raw data
Data analysis
Results analysis
Purification assessment report
Summary of relevant parameters
Why Choose Us?
Creative Enzymes is a biotechnology company that specializes in providing tool enzyme services. As a reliable supplier, Creative Enzymes offers its services with high quality and competitive price/performance ratio. Creative Enzymes is able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. Creative Enzymes has accumulated years of quintessence in the field of sequence-based cloning enzyme screening, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.
