Overview

Enzyme activity is measured in vitro under conditions that often do not closely resemble those in vivo. The objective of measuring enzyme activity is normally to determine the amount of enzyme present under defined conditions, so that activity can be compared between one sample and another, and between one laboratory and another. The conditions chosen are usually at the optimum pH, 'saturating' substrate concentrations, and at a temperature that is convenient to control. In many cases the activity is measured in the opposite direction to that of the enzyme's natural function. Nevertheless, with a complete study of the parameters that affect enzyme activity it should be possible to extrapolate to the activity expected to be occurring in vivo.

Factors affecting enzyme activity

• The low levels of enzyme activity
• Competition among the substrates and cofactors
• Weak response from the reporter group
• Low sensitivity of detection method
• Non-linear reaction rates
• Interactions with other components in the solution
• Possible enzyme inhibition by other ingredients

Sepcific DNA Modifying Enzymes Activity Assay Approaches We Offer

• Stopped assays

To measure the activity of an enzyme one must measure how much product is formed over a given time or, in some cases, how much substrate has been used up, which should be the same thing. Thus ideally a method for measuring either product or substrate in the presence of the other is required. There are many different approaches; this section will deal with what are known as 'stopped assays'.

• Continuous assays

The alternative to a stopped assay is a continuous one in which the progress of the reaction is followed as it occurs. Continuous assays are much more convenient in that the result is seen immediately, and any deviation the initial rate shows from linearity can be observed. On the other hand not all enzymes have an assay method that can be observed continuously.

Service Process

Our Features

• Creative Enzymes has established a proven enzyme technology platform for a variety of applications. We'll help you design the right DNA modifying enzyme service to aid your scientific research.
• Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in DNA modifying enzyme service.

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional DNA modifying enzyme service provider supporting a wide range of enzyme services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years of quintessence in the field of DNA modifying enzyme activity assay service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.