Overview

DNA modifying enzymes, such as DNA methyltransferases, DNA glycosylases and DNA demethylases play important roles in regulating gene expression and maintaining genomic stability. Accurate measurement of their activities is essential to better understand their functions in gene regulation and disease processes. Fluorometric assays are widely used to measure enzyme activity due to their high sensitivity, specificity and speed. The assay works by monitoring the fluorescence emission when a substrate or a product specifically modified with a fluorophore interacts with the enzyme.

Methods

Fluorometric assays have become popular in assessing the activity of DNA modifying enzymes due to their high throughput nature and sensitivity. Depending on the specific enzyme being studied, different fluorophores and substrates can be utilized. The following are some examples of fluorophores and substrates used for DNA modifying enzyme assays:

• Methyltransferases
• Glycosylases
• Demethylases

Sepecific Assay Service We Offer

We offer a comprehensive DNA modifying enzymefluorometric service, providing a reliable and accurate method for measuring enzyme activity. Our team has extensive experience with a variety of enzymes and fluorophores and can customize your assay to meet your specific needs. we offer you the following services according to your scientific needs:

• Assay optimization: We work with you to determine the best fluorophore and substrate combination for your specific enzyme of interest. We optimize assay conditions to ensure high sensitivity and accuracy.
• Enzyme Kinetics: We determine the Michaelis-Menten constant (K_m) and maximum velocity (V_max) for your enzyme to evaluate its substrate affinity and catalytic efficiency.
• Inhibitor Screening: We screen compounds to see if they can inhibit your enzyme of interest. This is useful for drug discovery and development.
• High-throughput screening: We can perform assays in 96-well or 384-well plates for high-throughput screening of large libraries of compounds.

Sample requirements

We require a minimum of 1 μg of purified enzyme or cell lysate per assay. We recommend that samples be frozen at -80°C until shipment.

Our Features

• Customized solutions: We offer customized solutions to meet the specific needs of our clients.
• Customer Satisfaction: Our team works closely with our clients to ensure customer satisfaction.
• Competitive pricing: We offer competitive pricing without compromising the quality of our services.

Deliverables

At the end of our DNA modifying enzyme fluorometric service, you will receive a detailed report of the results, including:

• A summary of the assay conditions and methods used
• Description of the enzymatic kinetics, including K_m and V_max values
• Plot of fluorescence versus time or concentration
• IC₅₀ values for any inhibitors screened
• Detailed protocol for experimental optimization and inhibitor screening

Why Choose Us?

Our DNA modifying enzyme fluorometric service provides a reliable and accurate method for measuring enzyme activity. Our team has extensive experience with a variety of enzymes and fluorophores and can tailor the assay to your specific needs. Through this service, we aim to provide our clients with valuable insight into the function of DNA modifying enzymes, enabling them to make significant contributions to the field of epigenetics and disease research.