Restriction enzyme folding is the physical process of translating a protein chain into its natural three-dimensional structure, usually the "folded" conformation of a protein with a biological function. The folding of many enzyme begins even during the translation of the polypeptide chain. Amino acid interactions produce a well-defined three-dimensional structure. The correct three-dimensional structure is essential for function, although some parts of a functional protein may remain unfolded. Failure to fold into the natural structure usually produces inactive proteins, but in some cases, misfolded proteins have modified or toxic functions. Denaturation of enzymes is the process of changing from a folded state to an unfolded state.

Figure 1. Results of enzyme folding.

Sepcific Restriction Enzyme Folding Analysis Approaches We Offer

Creative Enzymes offer a one-stop restraint enzyme folding analysis service that can meet your research needs for restriction enzyme research. Creative Enzymes can provide you with a variety of restraint enzyme folding analysis services.

Methods

• CD ComputaBio provides expertise to help identify the correct structural fold in the known enzyme structure of a target restriction enzyme by combining sequence map comparisons with multiple structural information.
• Our approach starts with the construction of a structural template database/library and then gradually replaces the sequences of known protein structures in the library with query sequences of unknown structures.
• An optimally designed scoring function allows comparison of the target sequences with each structural template. This process is repeated for all known 3D structures in the template database until the most suitable structure is found.
• The statistically most likely comparison is selected as the thread prediction, and a skeleton model of the target is constructed by placing the skeleton atoms of the target sequence at the locations of the comparison skeletons of the selected structural templates. Unlike performing only sequence comparisons, these methods take advantage of the additional information provided by the 3D structural information. The generated structural models are quality-validated and can be used in computer software or downstream experimental work such as enzyme engineering and drug design.

Service Process

• Creative Enzymes will complete your project on time and efficiently. We have professional after-sales service.
• Creative Enzymes works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
• Creative Enzymes has a proven, advanced technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.

Deliverables

• Raw data
• Data analysis
• Image and result analysis
• Results analysis
• Stability assessment report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional restriction enzyme folding analysis service provider, supporting a variety of sequencing services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. Creative Enzymes has accumulated years of quintessence in the field of enzyme folding analysis service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.