Molecular cloning involves the replication of DNA fragments, thereby elucidating manipulation studies. Most commonly, cloning is accomplished by inserting one or several DNA fragments of interest (also known as inserts) into a circular and self-replicating DNA fragment (also known as a plasmid vector). When the ligated plasmid/insert is introduced into the bacteria, the looped plasmid/insert replicates itself and produces hundreds of identical clones in each cell. To initiate the reaction, DNA restriction enzymes are used to identify and digest the plasmid vector and insert specific nucleotide sequences so that they can be easily ligated together by DNA ligase for further recombinant DNA production.
Creative Enzymes is an outstanding supplier and manufacturer that remains committed to research and experimentation in order to provide its customers with a comprehensive enzyme service and product line. In order to develop efficient enzyme tools to better support customer research, Creative Enzymes now offers custom cloning enzyme manufacturing service to its global customers. Creative Enzymes provides you with the following custom cloning enzyme manufacturing service to meet your scientific research needs:
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HiFi Pfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity. It also possesses the 3'-5' proofreading exonuclease activity. |
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Taq DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus, which consists of a single polypeptide with a molecular weight of approximately 94 kDa. |
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GC enhancer should be used with DNA polymerase to optimize PCR from complex templates, including GC-rich templates. |
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PCR Stimulants can be used to upregulate the sensitivity and specificity of GC/AT-rich templates or complex templates, especially for Pfu enzymes. |
Formats From restriction enzymes to cofactors to substrates, we can customize reagents to meet your needs. Our components can be custom sized and formatted to your choice.
In customized production, efficiency and timeliness of results are critical. Optimize your workflow needs with a wide range of customized options.
Creative Enzymes is a company that provides professional and comprehensive restriction enzyme activity assays service. We have years of experience to meet your specific project needs in using enzyme research to add value to your research projects. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.
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