Cloning Enzyme sequence confirmation is an important step in the process of recombinant DNA technology. It involves the enzymatic confirmation of the nucleotide sequence of a cloned gene segment, which allows researchers to verify the presence of the desired gene and ensures the accuracy of the cloning process. At Creative Enzymes, we offer a comprehensive cloning enzyme sequence confirmation service that uses state-of-the-art technology to provide reliable and accurate results.
Specific Services We Offer
At Creative Enzymes, we offer a range of cloning Enzyme sequence confirmation services to meet the specific needs of our clients. Our services include：
PCR-based sequencing: We use polymerase chain reaction (PCR) to amplify cloned gene fragments, and then sequence the amplified products using Sanger sequencing.
Next generation sequencing: We use next generation sequencing (NGS) technology to sequence the entire gene fragment of the clone.
Restriction enzyme analysis: We perform restriction enzyme digestion of the cloned gene fragments followed by agarose gel electrophoresis to confirm the expected fragment size.
Colony PCR: We perform colony PCR on bacterial colonies carrying the cloned gene fragment to confirm the presence of the desired insert.
Services Process
Sample preparation: Customers submit their bacterial cultures or plasmid DNA samples containing cloned gene segments to our lab.
PCR Amplification: We perform PCR amplification of the cloned gene fragment using primers specific to the target sequence.
Sequencing: We sequence the amplification products using Sanger sequencing or NGS technology and generate a consensus sequence.
Data analysis: Our team performs data analysis to confirm the presence of the desired gene fragment and verify its accuracy.
Methods
Our cloning Enzyme sequence confirmation service uses a combination of PCR-based sequencing and NGS technology to confirm the sequence of cloned gene fragments.
For PCR-based sequencing, we first design primers targeting the cloned gene fragments and use them to perform PCR amplification. The obtained PCR products were purified and sequenced using Sanger sequencing.
For NGS, we sequenced the entire cloned gene fragment using a technology platform that generated millions of reads and assembled them into a consensus sequence.
Sample Requirements
To ensure optimal results, the following sample types and quantities are required for our cloning enzyme sequence confirmation service:
Bacterial culture: 1-5 ml of bacterial culture containing cloned gene fragments at a concentration of at least 10^6 CFU/ml.
Plasmid DNA: 1-5 µg of plasmid DNA containing cloned gene fragments at a concentration of at least 100 ng/µL.
Deliverables
Sequencing Report: A detailed report outlining the nucleotide sequence of the cloned gene segment, including any identified mutations or errors.
Quality Control Report: A summary of the quality control measures performed during the sequencing process to ensure accurate and reliable results.
Why Choose Us?
Comprehensive Services: Our range of services includes PCR-based sequencing, NGS, restriction enzyme analysis and colony PCR, ensuring that we have the perfect solution to meet your unique needs.
Fast turnaround times: We understand the importance of timely results, which is why we offer fast turnaround times without sacrificing quality.
Competitive pricing: We offer competitive pricing for our services, making us a cost-effective choice for your clonase sequence confirmation needs.
