Radioactivity assays

DNA modifying enzymes are used to manipulate DNA molecules and have a wide range of applications in biotechnology, including gene editing, cloning and PCR amplification. One of the most common methods of measuring the activity of these enzymes is by radioactive assay. Radioactivity assays are based on the incorporation of radioactive nucleotides into DNA during the enzyme reaction. the amount of radioactivity incorporated is proportional to the activity of the enzyme, which can be quantified by measuring radioactivity using scintillation counting. creative Enzymes offers a full range of high quality DNA modifying enzymes and radioactivity assays to meet the needs of researchers in a variety of fields.

Methods

Radioactive assays measure the activity of DNA modifying enzymes by monitoring the binding of radioactive nucleotides in DNA. Typically, radiolabeled dNTPs (deoxynucleoside triphosphates) are used in the assay, and they are available in alpha-, beta-, or gamma-emitting isotopes. The enzymatic reaction is carried out in the presence of radionucleotides and the reaction products are separated by gel electrophoresis. The radiolabeled DNA bands are then observed by autoradiography or fluorescence imaging, and the amount of radioactivity bound to the DNA is measured by scintillation counting.

Sepecific Radioactive Assay Service We Offer

Creative Enzymes offers a comprehensive range of radioactive assays to analyze DNA modifying enzyme activity. Our services include:

• Custom Assay Design: Our experts can work with you to design a custom assay to meet the specific requirements of your research project.
• High quality enzymes: We offer a wide range of high quality DNA modifying enzymes including DNA polymerases, restriction enzymes, ligases and exonucleases.
• State-of-the-art equipment: We use the most advanced equipment and technologies to ensure accurate and reliable results.
• Fast turnaround times: Our streamlined processes allow us to provide fast turnaround times without compromising quality.
• Competitive pricing: We offer competitive pricing for all of our services with no hidden costs or fees.

Service Process

Sample Requirements

We require a minimum of 1 µg of DNA substrate per radioactive assay. DNA substrates can be linear or circular, and either plasmid or genomic DNA can be used. We also require detailed information about the enzyme being assayed, including enzyme source, concentration and reaction conditions.

Applications

Cloning: DNA modifying enzymes are used in cloning to cut and paste DNA molecules to create recombinant DNA molecules.

PCR amplification: DNA polymerases are used in PCR amplification to amplify specific DNA sequences.

DNA sequencing: DNA modifying enzymes are used in DNA sequencing to produce high quality sequence data.

Our Features

• High quality and reliable results backed by our extensive experience and stringent quality control measures.
• Solutions tailored to your specific study requirements.
• Ongoing technical support to ensure successful implementation of the assay in your study.
• Competitive pricing and fast turnaround times.

Deliverables

• Assay protocol
• Sample preparation details
• Enzyme activity measurements
• Data analysis and interpretation

Why Choose Us?

Creative Enzymes is a professional NA modifying enzymes radioactive assay service provider supporting a wide range of enzyme services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years of quintessence in the field of DNA modifying enzymes radioactive assay, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects.