Background

The crosslinking process can be performed either in vivo or in vitro, which is used depends on the needs of the project. The difference between the two is that cloning enzyme crosslinks in its natural state during in vivo crosslinking, whereas for in vitro crosslinking, denaturation may occur.

Features of Crosslinking Interaction Analysis

• In vivo transient interactions can be analyzed.
• In vitro protein interactions with low affinity can be analyzed.
• High detection sensitivity.

Our Workflow

1. Design of crosslinking methods and crosslinker types according to your specific project needs: in vivo/in vitro.
2. Optimization of reaction conditions, targeted to the sample characteristics and project needs.
3. Cell lysis, CoIP.
4. Protein immunoblotting/mass spectrometry analysis (depending on project needs).
5. Data analysis and report delivery.

One-Stop Service

• Just place your order - send your samples
• Creative Enzymes is a one-stop shop for:

Related Service

• Mass Spectrometry Analysis

Creative Enzymes provides protein interaction analysis services and subsequent mass spectrometric identification of proteins/protein mixtures in purified samples such as IP, Co-IP samples and GST fusion protein Pull-down based on liquid mass spectrometry (LC-MS/MS).

• Protein Interaction Analysis by CO-IP Immunoprecipitation

Creative Enzymes provides high quality Co-IP immunoprecipitation protein interaction analysis services to our clients. Our optimized protocols for sample protein extraction, expression and purification ensure high quality bait and prey proteins. In addition, we offer custom methods for characterizing potential interacting proteins.

• GST Pull-Down Protein Interaction Analysis

GST pull-down fusion protein deposition technique protein interaction analysis focuses on the study of strong or stable protein interactions in vitro; allows the identification of possible direct interactions between two known proteins of interest; and looks for unknown proteins that may have an interaction with the target protein.

Our Features

• Creative Enzymes has established a proven enzyme technology platform for a variety of applications. We'll help you design the right cross-linking method to aid your scientific research.
• Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in cross-linking method.   

Service Process

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional custom tool enzyme service provider, supporting a variety of services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. Creative Enzymes has accumulated years of quintessence in the field of tool enzyme service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.