Restriction Enzyme Mutation Site Prediction

Enzyme engineering plays a vital role for industry and research, but the expensive and time-consuming evaluation of mutants in the laboratory limits the number of variants that can be explored. As a result, researchers have begun to investigate the use of machine learning to predict mutations in enzymes. Creative Enzymes provides you with a variety of restriction enzyme mutation site prediction service to meet your scientific research needs.

Sepcific Restriction Enzyme Mutation Site Prediction Approaches We Offer

Restriction Enzyme Mutation Site Prediction

  • Creative Enzymes provides predictive methods to understand not only genomic sequence variation during the evolution of organisms, but also to provide valuable information about genetic variation in genetic diseases and the emergence of drug resistance in cancer and infectious diseases.
  • We disrupt protein function through the effects of mutations on gene expression and regulation, and by regulating protein stability or interfering with interactions with proteins, nucleic acids, small molecule ligands and other biomolecules. Experimental methods for understanding the differences between wild-type and mutant proteins are the most accurate.
  • Computational tools for predicting the effects of mutations can provide useful information more quickly.

Methods

We focus on two widely used structure-based methods:

  • Site Directed Mutator (SDM)

SDM is a statistical method for analyzing amino acid substitutions, the fixed-point mutator (SDM) is a computational method that analyzes changes in amino acid substitutions that occur in specific structural environments tolerated in homologous protein families with known 3-D structures and converts them into tables of substitution probabilities. These tables are used as quantitative measures for predicting protein stability after mutation.

  • Mutation Cutoff Scan Matrix (mCSM)

The mCSM is based on features to represent the wild-type structural environment and machine learning to predict the effect of mutations on protein stability.

Workflow

Restriction Enzyme Mutation Site Prediction

  • Preparation
  • Influence of different embeddings
  • Performance of the different machine learning algorithmsEffect of dataset size
  • Effect of dataset split
  • General prediction performance
  • Sensitivity analysis
  • Mutation effect prediction

Service Process

Service Process

Our Features

  • We have accumulated many years of essence in the field of integrating restriction enzyme mutation site prediction service, helping customers to speed up project development and improve the overall success rate of the project.
  • At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.

Deliverables

  • Related results raw data
  • Data analysis
  • Image and result analysis
  • Results analysis
  • Stability assessment report
  • Summary of relevant prediction parameters

Why Choose Us?

Creative Enzymes is a company that provides professional and comprehensive restriction enzyme mutation site prediction service. We have years of experience to meet your specific project needs in using enzyme research to add value to your research projects. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.

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