Overview

DNA modifying enzymes play a key role in the regulation of gene expression and DNA repair. Quantifying the activity of these enzymes is essential for understanding their biological functions and developing therapeutic interventions. Mass spectrometry (MS) assays have become a powerful tool for quantifying enzyme activity with high sensitivity and specificity.Creative Enzymes offers comprehensive DNA modifying enzyme mass spectrometry to help researchers measure enzyme activity and characterize inhibitors.

Methods

Several MS-based strategies are available for quantifying the activity of DNA modifying enzymes, including:

Substrate targeted MS assay: This assay measures the conversion of a labeled nucleotide substrate by the enzyme of interest into its modified form. The modified nucleotides are then detected and quantified by mass spectrometry.

Product-targeted mass spectrometry: This assay measures the removal of chemical groups from the substrate by the enzyme of interest. The resulting product is then detected and quantified by mass spectrometry.

Stable isotope labeled MS assay: This assay uses a stable isotope to label the substrate or product of the enzyme of interest, allowing for sensitive and accurate detection by MS analysis.

Sepecific Assay Service We Offer

• Creative Enzymes offers a range of MS-based solutions for measuring the activity of DNA modifying enzymes, including custom assay design, optimization, validation and data analysis.
• Our team of experts works closely with our customers to develop customized solutions to meet their specific research needs.
• We offer a variety of mass spectrometry platforms including electrospray ionization (ESI), matrix-assisted laser desorption/ionization (MALDI), and time-of-flight (TOF) mass spectrometers.

Sample requirements

We require a minimum of 1 μg of purified enzyme or cell lysate per assay. We recommend that samples be frozen at -80°C until shipment.

Our Features

• High quality and reliable results backed by our extensive experience and stringent quality control measures.
• Solutions tailored to your specific study requirements.
• Ongoing technical support to ensure successful implementation of the assay in your study.
• Competitive pricing and fast turnaround times.

Deliverables

We provide our clients with detailed reports that include all experimental procedures and results obtained. The report includes a summary of the assay design, optimization, validation and data analysis.

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes provides high quality DNA modifying enzyme MS assays to help researchers measure enzyme activity and identify inhibitors. Our comprehensive services include custom assay design, optimization, validation and data analysis. We use validated and optimized protocols to ensure accurate and reproducible results. Our experienced team of professionals can assist with all aspects of the assay, from experimental design to result interpretation. We are committed to delivering high-quality services that meet our clients' specific needs and exceed their expectations.