Overview

DNA ligases are enzymes that play an important role in the replication, repair and recombination of DNA. As such, they are widely used in molecular biology research, genetic engineering and drug development. One of the most widely used DNA ligases is the T4 DNA ligase. However, not all laboratory experimental needs can be met by commercially available DNA ligases. Therefore, some researchers may require a custom DNA ligase with specific properties. Although DNA ligases are readily available from commercial sources, custom formulations are sometimes required to meet specific experimental needs. This is where custom DNA ligases come into play.

Sepecific Services We Offer

Our custom DNA ligase manufacturing service is designed to meet our customers' specific requirements and produce ligases with high affinity, reproducibility and purity.

Manufacturing Process

We use high quality materials and advanced manufacturing techniques to ensure that our custom DNA ligase products have excellent amplification efficiency and stability. The production process for our custom DNA ligases is as follows:

• Prepare equipment, reagents and raw materials according to customer specifications.
• Specify customer requirements for the DNA ligase, including activity, concentration, etc.

We then perform the following steps:

• Expression and purification: We first select a suitable expression host and clone the corresponding DNA sequence. Next, we express the DNA ligase protein and purify it from the host using our proprietary purification process.
• Quality Control: We perform multiple quality control processes to ensure that our custom ligases meet our customers' specifications, including activity assays, SDS-PAGE analysis, and purity analysis.
• Packaging and Delivery: We package our custom DNA ligase products to the highest industry standards and ship them to our customers via the delivery method of choice.

Methods

• Site-specific mutagenesis: Mutagenesis can be used to introduce specific mutations in gene sequences, allowing DNA ligases with specific properties to be tailored for specific applications.
• Gene Synthesis: Creative Enzymes synthesizes gene sequence fragments using high quality synthesis techniques.
• Cloning and Expression: We can clone target gene sequences into our vector systems to expand the high expression of custom sequences.

Deliverables

• For custom DNA ligase products, we work closely with our customers to ensure that the final product meets their specific needs.
• Upon completion, we provide a detailed product characterization report, as well as any other data that may be useful.

Why Choose Us?

Creative Enzymes offers a comprehensive custom DNA ligase manufacturing service that can help laboratories and companies in further scientific research and development. We are dedicated to delivering high-quality, customized DNA ligase products that meet the specific demands and requirements of our clients. If you are interested in our custom DNA ligase manufacturing services or have any questions, please don’t hesitate to contact us. We’re here to help!