Creative Enzymes is a leading provider of enzyme-related services and products, specializing in the production, purification, modification and characterization of enzymes for a variety of biotechnology applications. One of our core products is our cloning enzyme PCR amplification service, which we offer to researchers and customers around the world. Our cloning enzyme PCR amplification service is designed to help researchers clone genes of interest efficiently and accurately. With extensive experience in molecular biology techniques and state-of-the-art equipment, we can provide customized, reliable solutions to meet your specific research needs.
Specific Services We Offer
Our cloning enzyme PCR amplification services include the following services:
Gene Amplification: We amplify genes of interest from genomic or cDNA templates using high-fidelity DNA polymerase and optimized PCR conditions.
Cloning: We utilize C-type cloning enzyme technology to efficiently generate blunt-end PCR products suitable for ligation to multiple cloning sites in vectors.
Vector Construction: We provide custom vector design and construction services to ensure maximum expression levels and compatibility with downstream applications.
Sequence Validation: We perform sequence validation to ensure that the final constructs match the desired sequence with high fidelity.
Sample Requirements
Genomic DNA or cDNA sample: A purified genomic DNA or cDNA sample should be provided to us.
Gene-specific primer sequences: You should provide us with the sequences of the gene-specific primers required for PCR amplification.
Workflow
Our cloning enzyme PCR amplification service uses a modified PCR amplification protocol that incorporates a unique set of enzymes and reaction conditions. The process consists of the following steps:
Template preparation: Genomic DNA or cDNA is extracted from the sample of interest and purified using standard procedures.
PCR Amplification: A pair of gene-specific primers is designed and the target gene is amplified using a high-fidelity DNA polymerase in a standard PCR reaction.
Blunt End Generation: PCR products are treated with a C-type cloning enzyme mixture to generate blunt ends suitable for ligation to multiple cloning sites of the vector.
Ligation: The PCR product is then ligated to a vector using standard molecular biology protocols such as Gibson Assembly or T4 DNA ligase.
Transformation: The ligation reaction is transformed into competent cells and selected on agar plates.
Sequence verification: Colonies containing the desired construct are selected and the sequence of the final construct is verified by sequencing analysis.
Deliverables
Purified Plasmid DNA: We will provide you with purified plasmid DNA containing the cloned gene of interest.
Sequencing data: We will provide you with sequencing data to verify the accuracy of the cloned gene sequence.
Our Features
State-of-the-art equipment: Our facilities are equipped with state-of-the-art equipment, which allows us to provide you with high-quality results and reliable data.
Quality Control: We employ strict quality control measures at every step of the service process to ensure accuracy and repeatability.
Cost-effective solutions: We provide cost-effective solutions without compromising the quality of our services.
Why Choose Us?
Creative Enzymes' cloning enzyme PCR amplification services are a reliable, flexible and cost-effective solution for gene cloning and protein expression studies. With our expertise and rigorous quality control processes, we can deliver high-quality results tailored to your specific research needs. Contact us today to learn how we can help accelerate your research and discovery efforts!
