Polynucleotide kinase (PNK), a member of the 5'-kinase family, is a functional biological enzyme specifically involved in the process of DNA phosphorylation.The normal function of PNK also plays an important role in restoring 5'-phosphate-dependent DNA and maintaining genetic integrity. Conversely, overexpression or blockade of PNK activity causes abnormal levels of nucleic acid phosphorylation and triggers various human diseases, such as diseases of neurodegeneration, loom syndrome, Werner syndrome and Rosemond-Thomson syndrome.
Why is DNA glycosylase testing necessary?
Inhibition of T4 PNK activity improves the efficiency of γ-radiotherapy in the treatment of somatic cancers, suggesting that this kinase is a promising target for drug design and discovery. Therefore, monitoring PNK activity and its inhibitors is an effective way to develop therapeutic regimens and to study the process of nucleic acid metabolism.
Sepecific Polynucleotide Kinase Detetion Service We Offer
Creative Enzymes offers a fluorescent sensor based on target cycle amplification combined with HCR for the detection of carcinoembryonic antigen (CEA) and PNK activity.
Creative Enzymes has designed a cascade signal amplification fluorescent sensor based on primer template conversion to detect PNK activity. In cascade amplification, the targeting dependence of the template allows for low background and low detection limits.
Creative Enzymes' nucleotide signal amplification technology, combined with fluorescence detection, is used to quantify DNA modifying enzymes.
Creative Enzymes offers fluorescent methods that rely on external labeling with fluorophores and bursting agents for homogeneous assays and are suitable for high throughput, but have relatively small signal windows and complex and expensive substrate probe designs.
Service Process
Our Capabilities
Advanced technology platforms: We are equipped with various technology platforms to accuratelyj detect DNA glycosylase.
Accuracy and reproducibility: We provide accurate and detailed results regarding sample purity and homogeneity, thus saving time wasted on bad samples.
Overall Solutions
As a global expert company in tool enzyme services, Creative Enzymes offers you a time-saving service. Our PhD team synthesizes everyday genes and sequences from any source and provides the right DNA modifying enzyme related work for your needs.
Our Features
Creative Enzymes works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
Creative Enzymes has a proven, advanced polynucleotide kinase detetion platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.
Deliverables
Results raw data
Data analysis
Results analysis
Summary of relevant parameters
Why Choose Us?
Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in polynucleotide kinase detetion service. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.
