Overview

Enzyme kinetics is the study of the chemical reactions that are catalyzed by enzymes. In enzyme kinetics, technicians measure reaction rates and investigate the effects of the varying conditions of the reaction. Studying an enzyme's kinetics can reveal the catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled, and how a drug or an agonist might inhibit the enzyme.

Sepcific DNA Modifying Enzymes Kinetics Assay Approaches We Offer

• Optical methods

We use different analytical techniques in order to capture the changes in the concentration of the substrate or product over a defined period of time. Optical methods, especially UV-Vis spectrophotometry, are particularly well suited for this task. Here, the absorbance is measured at a defined wavelength - either continuously or at a defined point in time. The advantage of continuous measurement is that the entire process can be monitored. In this way, the behavior of the assay can be monitored for deviations, and the linear range is easily defined. The enzyme activity can then be calculated based on the change in absorbance per unit time.

• Measurement process

For the measurement process, we determine the appropriate environmental parameters (e.g. temperature and pH). Their consistency throughout the experiment should be guaranteed, as they can severely affect enzyme activity. Parameters to be defined include the measurement wavelength, temperature and measurement duration and, if applicable, the defined measurement interval. Accurate pipetting and careful mixing are essential. In addition, care must be taken to start measurements immediately after the addition of the final reagent.

Service Process

Our Features

• We have accumulated many years of essence in the field of DNA modifying enzyme, helping customers to speed up project development and improve the overall success rate of the project.  
• At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.
• Creative Enzymes has in-depth cooperation with many biology companies and research institutes, and has extensive experience in providing them with high-quality products and services.

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional DNA modifying enzyme service provider, supporting a variety of enzyme-related services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. Creative Enzymes has accumulated years of quintessence in the field of DNA modifying enzymes kinetics assay service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.