Overview

DNA methyltransferases are an important class of enzymes that are able to add methyl groups to certain sites, thereby altering the epigenetic properties of DNA. This enzyme is widely found in living organisms and plays an important role in several life processes such as cell proliferation, development, and immune response. Therefore, the study of DNA methyltransferases is also of great scientific research value.

Sepecific Assay Service We Offer

We offer services related to the determination of DNA methyltransferase activity. Our laboratories are equipped with advanced equipment and professional technicians to provide you with accurate and reliable results, and to deliver the results in a timely manner according to your needs. We can provide you with the following services:

• DNA methyltransferase activity determination

We can determine DNA methyltransferase activity using a variety of methods, such as radioisotope labeling, fluorescent labeling, and molecular biology methods. We can select the appropriate method according to your needs to ensure the accuracy and reproducibility of the assay results.

• Measurement of enzyme kinetic parameters

We can measure the enzyme kinetic parameters of DNA methyltransferase, such as the maximum catalytic rate and affinity constant, to understand the catalytic mechanism and reaction kinetic properties of the enzyme. This will provide you with more in-depth research data and can provide a valuable reference for your subsequent experimental design.

• Inhibitor Screening

We can use enzyme activity assays to screen different types of compounds for their role in DNA methyltransferase inhibitors. In addition, we can conduct pharmacodynamic studies to support your anticancer drug research according to your needs.

Methods

We offer various methods to measure DNMT activity, such as

• Radioactive labeling
• Fluorescent labeling methods
• Molecular biology techniques

We select the appropriate method according to your needs to ensure accurate and reproducible results. For example, we can use radiolabeling methods to detect the incorporation of [3H]-labeled SAM into DNA by DNMT, which allows us to measure the enzymatic activity of DNMT. Alternatively, we can use molecular biology techniques, such as bisulfite sequencing, to determine the methylation pattern of DNA.

Sample requirements

We require a minimum of 1 μg of purified enzyme or cell lysate per assay. We recommend that samples be frozen at -80°C until shipment.

Applications

DNMT assay is widely used in various fields, such as :

The results of DNMT assay can provide valuable insights into the regulation of gene expression and contribute to the development of new therapies for diseases.

Deliverables

• We will provide detailed reports, including experimental methods, interpretation of results and statistical analysis.
• We also pay great attention to the accuracy and reliability of the data, so we will carefully check and verify all the data to ensure that you get high quality data.
• We promise to complete the test within the time you specify and send the results to you in time.

Why Choose Us?

Creative Enzymes is equipped with state-of-the-art equipment and cutting-edge technology to provide accurate and reliable results tailored to your specific needs. Our team of experienced scientists and technicians with extensive expertise in DNMT assays are committed to providing high quality services to meet your research needs.