The cloning donor immunoassay (CEDIA) is a competitive homogeneous enzyme immunoassay. The assay uses two component fragments of the enzyme, each of which is inactive. Under the appropriate conditions in solution, these fragments can spontaneously reassemble to form the active enzyme. To be used in the biochemical assay, one of the enzyme fragments is linked to the analyte of interest. The analyte-enzyme-fragment-adhesive is still able to reassemble with other enzyme fragments to form the active enzyme. Creative Enzymes provides you with a variety of cloning enzyme service to meet your scientific research needs.

Principle

The reaction mode of CEDIA is a competitive method, and the principle of the assay is that the antigen and ED-labeled antigen in the specimen compete with the specific antibody for binding, forming two antigen-antibody complexes. the ED-labeled antigen can no longer bind to the EA after binding to the antibody due to spatial blockage. After reaction equilibrium, the remaining ED-labeled antigen binds to the EA to form an active enzyme. The enzyme activity is determined by adding substrate and the magnitude of the enzyme activity is proportional to the antigen content of the specimen. CEDIA is mainly used for the determination of drugs and small molecules.

Application

The ED-labeled antigen is no longer able to bind to the EA after binding to the antibody due to spatial site blocking. After the reaction equilibrium, the remaining ED-labeled antigen binds to EA to form an active enzyme. CEDIA is mainly used for the determination of drugs and small molecules.

Service Process

Where We are Heading

As a company focused on tool enzymes, we strive to make our quality enzyme solutions available to any company and institution in any field of research and branch of industry. When you need cloning enzyme donor immunoassay service to make your products and processes faster, more cost effective, more environmentally friendly and more innovative, our goal is to be your first port of call.

Our Features

• We have accumulated many years of essence in the field of cloning enzyme donor immunoassay service, helping customers to speed up project development and improve the overall success rate of the project.
• At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.
• Creative Enzymes has in-depth cooperation with many biology companies and research institutes, and has extensive experience in providing them with high-quality products and services.

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Purification assessment report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a company that provides cloning enzyme donor immunoassay service. We have years of experience to meet your specific project needs. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.