Endotoxin, also known as LPS, originates from the cell membrane of Gram-negative bacteria and is responsible for their organization and stability. As cells die, grow and divide, endotoxin is continuously released into the environment. Endotoxins do not act directly on cells or organs, but rather release a range of pro-inflammatory mediators such as tumor necrosis factor (TNF), interleukin (IL)- 6, and IL-1 through activation of the immune system, particularly through monocytes and macrophages. endotoxins are therefore almost ubiquitous and cause a wide variety of pathophysiological effects. Creative Enzymes services, including method development, validation, transfer and method development, help you accelerate your drug development process based on your every need!

Methods

• The RPT: Rabbit Pyrogen Test
• The MAT: Monocyte Activation Test
• The LAL Test or Limulus Amebocyte Lysate Test
• The rFC: Recombinant Factor C assay

Custom Cloning Enzyme Endotoxin Detection Approaches We Offer

• Endotoxin Testing Services

Creative Enzymes offers sensitive and accurate cloning enzyme endotoxin assays.

• Endotoxin Removal Services

Creative Enzymes offers cloning enzyme endotoxin removal services to effectively reduce endotoxin levels in your products or other instruments.

• LPS Analysis Services

Creative Enzymes offers LPS analysis services to assist in the study of bacterial pathogenesis.

Service Process

Advantages

• Guidance for industrial process improvement.
• Easy and quick : 2 days advisory mission from our endotoxins experts.
• Adapted to your need: First contact with conference call, review of your data, complete report with advice and practical solutions, ending conference call to review the report.
• Benefit: A complete review of your process and the possible solutions available to endotoxins detection.

Our Features

• At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.
• Our bioinformatics team constantly strives to optimize the pipeline to deliver biologically meaningful results in a short around time.
• Our flexible and communicative team works closely with you to adapt to your analytical requirementse.
• We help you shape the methodological part of your manuscripte.
• We back up our in-house servers daily to protect your data.

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Purification assessment report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes offers a one-stop shop for cloning enzyme endotoxin assays, including the preliminary steps of gene synthesis and enzyme expression, as well as subsequent endotoxin assays.Creative Enzymes' endotoxin assay services are certified to yield products that meet the stringent requirements of downstream animal and cellular assays. With years of experience, Creative Enzymes works closely with customers throughout the purification process, solving technical challenges and reflecting the target process, Creative Enzymes is your best choice for enzyme purification involving endotoxin removal.