Overview

DNA cloning is a genomic manipulation technique that allows target DNA sequences to be cloned into vector DNA and replicated in cells, thereby amplifying the number of target DNA sequences. Restriction enzymes are important tools in DNA cloning that allow selective cloning of target DNA sequences by cutting specific DNA sequences. Therefore, restriction enzyme DNA cloning services are widely used in modern biomedical research, agriculture and environmental science.

Sepecific Services We Offer

We offer DNA cloning services with restriction enzymes, enabling researchers to obtain cloned DNA sequences quickly and efficiently. Our team of experts has extensive experience in DNA cloning and uses state-of-the-art technology to ensure the highest quality results. Our service process is as follows:

• Target DNA sequence specificity identification: We first need to determine if the target DNA sequence is eligible for restriction enzyme cloning to identify the appropriate restriction enzyme as well as to select the appropriate restriction enzyme cleavage site.
• DNA cleavage: We will cleave the target DNA using the appropriate restriction enzyme to cut the target DNA sequence to an appropriate size for cloning.
• DNA Library Construction: We will clone the target DNA using the appropriate vector and restriction enzymes to construct your desired DNA library.
• Targeted mutation screening: We can subclone the target DNA sequence and screen for targeted mutants using genetic engineering techniques such as PCR and restriction enzyme shearing.
• DNA amplification and refinement: We will apply PCR amplification techniques for your desired DNA fragments to expand the number of cloned target DNA sequences, and perform the necessary purification and refinement.

Workflow

Once we receive your sample, we will digest it with the appropriate restriction enzymes to create ligated ends ready for ligation. Next, we will clone the digested DNA fragment into the vector of your choice, transform the vector into competent cells, and select colonies carrying the correct constructs. Finally, we will verify the sequence of the cloned DNA fragment by Sanger sequencing to confirm its identity.

Sample Requirements

• Samples should be high quality double-stranded DNA.
• The sample should be of sufficient concentration and amount to ensure the accuracy of the cloning results.
• Samples should be clean, pure, and free of contaminants and inhibitors.

Deliverables

• Detailed information on sample preparation and testing.
• Information and analysis of cloning data.
• A full report of the cloning results, including data analysis and biological profile.
• We can also provide you with further data analysis and consulting services if required.

Why Choose Us?

Creative Enzymes offers the highest quality restriction enzyme DNA cloning services and is committed to providing the most powerful assistance and support for your research efforts. Our team of technical professionals and extensive experience can provide you with comprehensive services and ensure that your needs and expectations are met. Whatever your questions or requests about our services, we will listen attentively and strive to provide you with the highest quality service.