Restriction enzymes mainly refer to type I restriction endonucleases, which can cut DNA at specific sites of DNA. They are mainly ligases, polymerases, methylesterases, phosphatases, phosphokinases, nuclear jaw enzymes, etc. They can catalyze the ligation, polymerization, site-specific modification, ligation or removal of phosphate groups of DNA or RNA. Creative Enzymes provides you with a variety of restriction enzyme purification service to meet your scientific research needs.

Sepcific Restriction Enzyme Purification Service Approaches We Offer

Creative Enzymes uses renewable resources for the isolation and purification of restriction enzymes. We use recombinant technology more often. In particular, the production of certain restriction enzymes, which are low in natural substances and difficult to purify, can be greatly improved by using recombinant technology.

Methods

• Low temperature centrifugation

After fragmentation, the lysate can be removed by low temperature ultracentrifugation to remove a small amount of unbroken cells, broken debris and ribosomes and other large particles to obtain a clarified supernatant, often called crude enzyme preparation, which can be used for further purification.

• Removal of nucleic acids

Since the presence of nucleic acids and their fragments in the crude enzyme solution can interfere with further purification by ion exchange column chromatography, nucleic acids are usually removed before column chromatography. We commonly use the following methods:

• Streptomycin sulfate
• Polyethyleneimine method
• ammonium sulfate stepwise precipitation method, etc.
• Column chromatography purification

The above purification of crude extract can be called preliminary purification, while the purification of column chromatography can be called precision purification, most of the purification of tool enzymes need 2~5 times of column chromatography purification to achieve the purity requirements for genetic engineering, column chromatography is the key step of tool enzyme purification. Column chromatography mainly includes.

• Ion exchange chromatography
• Affinity chromatography
• Adsorption chromatography
• Gel filtration

Advantages

• State-of-the-art candidate processing and tracking.
• With multiple subsets of libraries designed for specific screening needs.
• Each candidate is up-to-date, less than 2 years old at the time of selection.
• Candidates are rigorously screened to exclude undesirable features.
• All candidate compounds or building blocks have test specifications.

Service Process

Our Features

• Creative Enzymes will complete your project on time and efficiently.
• Creative Enzymes works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.

Deliverables

• Related results raw data
• Data analysis
• Image and result analysis
• Results analysis
• Activity assessment report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional restriction enzyme purification service provider, supporting a variety of enzyme-related services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. Creative Enzymes has accumulated years of quintessence in the field of restriction enzyme purification service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.