Overview

DNA modifying enzymes play a pivotal role in the regulation of gene expression and the modification of DNA structure. Scientists have developed various strategies to improve the catalytic efficiency, specificity and stability of these enzymes. Directed evolution is a powerful protein engineering technique that has been extensively utilized in this field. It is a rapid and effective method to generate variants with desired properties by mimicking natural selection. Creative Enzymes provides you with a variety of directed evolution service to meet your scientific research needs.

Sepecific Directed Evolution Service We Offer

At Creative Enzymes, we offer customized DNA modifying enzyme directed evolution services to meet the specific needs of our clients. Our services include:

• DNA methyltransferases (DNMTs) and demethylases (DMRs): We can engineer these enzymes to improve their specificity and activity in targeting DNA methylation or demethylation.
• Histone acetyltransferases (HATs) and deacetylases (HDACs): We can engineer these enzymes to improve their catalytic efficiency and selectivity for histone substrates.
• Endonucleases and ligases: We can design these enzymes to improve their cleavage or ligation efficiency, specificity and stability.
• Polymerases: We can engineer polymerases to improve their processing efficiency, fidelity or speed, and introduce new features such as unnatural base binding.

Service Process

Directed evolution is a Darwinian principle-based approach for designing proteins that can perform better than their wild-type counterparts. The process consists of four basic steps: library creation, selection/screening, amplification and characterization.

We use state-of-the-art technology to generate diverse libraries with high quality and fidelity. We have a wide range of assays and screening methods that are customized to meet the specific needs of our clients. We have established robust protein expression and purification protocols. We can deliver large quantities of highly purified proteins in a short period of time.

Applications:

• Epigenetic research: By designing DNMTs, DMRs, HATs, HDACs and other epigenetic enzymes, researchers can gain insight into the regulation of gene expression and the pathogenesis of various diseases, such as cancer and neurological disorders.
• Industrial Biotechnology: Engineered DNA-modifying enzymes can be used in various industrial processes such as bioremediation, biosynthesis and biofuel production.

Our Features

• Customizable Solutions: We offer customizable solutions to meet the unique needs of our customers.
• Fast turnaround times: Our efficient screening and optimization process ensures fast turnaround times for our customers.
• Competitive pricing: We offer competitive pricing without sacrificing quality.

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes provides top-notch DNA modification enzyme directed evolution services to assist researchers in exploiting the full potential of these enzymes. Our solutions cover a wide range of applications, from epigenetic research to drug discovery and industrial biotechnology. We have established comprehensive service processes and capabilities to provide personalized services to meet the specific needs of our clients.