Background

Single enzyme mass spectrometry identification/analysis and sequence analysis of single enzymes are frequently encountered problems in biological research. Mass spectrometry-based acquisition of enzyme sequence information and mass spectrometry identification by mass spectrometry, mass spectrometry identification based on protein profiling by LC-MS. Common analyses include: protein molecular weight determination by mass spectrometry, N-terminal and C-terminal sequencing of proteins, protein sequence analysis and post-translational modification site analysis, etc.

Analysis Approaches We Offer

1. Protein mass spectrometry/mass spectrometry identification of purified enzymes.
2. Identification of protein dots, protein glue strips by mass spectrometry, identification of protein silver-stained glue mass spectrometry, identification of glue dots/strips samples stained with test stain/fluorescence/Zn/Cu²⁺ types.
3. Protein identification/analysis of protein samples in simple mixture samples such as pull-down protein spectra identification, IP-MS reciprocal protein identification for IP protein interactions, Co-IP mass spectrometry to verify reciprocal proteins, immunoprecipitation combined with mass spectrometry verification.
4. Protein profiling/protein identification of unknown enzymes in complex samples. Even very low concentration enzyme samples may be identified by protein profiling.

Workflow

• Sample pre-treatment

First, the enzyme samples are reduced with dithiothreitol (DTT) and alkylated with iodoacetamide (IAA). Then the samples are digested with trypsin, and generally the samples are broken after lysine and arginine residues. Finally, the peptide fragments are obtained by lyophilization.

• On-machine analysis

The samples were re-solubilized with the A-phase of NanoLC and then uploaded for analysis under NanoLC-MS/MS system.

• Database search

The proteomic database of the corresponding species is downloaded from UniProt according to the source of the sample species, and the data obtained from the LC-MS is matched with the data in the database using software such as MaxQuant, Proteome Discoverer, Peaks, etc.

• Report issuance

The identification report will include details of the sample pre-processing steps, chromatography-mass spectrometry platform parameters, and a list of the proteins and peptides identified. The raw mass spectrometry data will be made available on a web site.

Our Features

• Creative Enzymes works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
• Creative Enzymes has a proven, advanced mass spectrometry identification technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.

Service Process

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in cloning enzyme mass spectrometry identification. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.