Creative Enzymes provides high quality cloning enzyme library construction services. Our state-of-the-art technology and experienced team ensure that we provide high quality sequencing libraries to accelerate your research and development projects. We offer a range of services to meet the needs of our diverse clients in the fields of drug discovery, gene therapy and synthetic biology.
Specific Services We Offer
DNA extraction and purification: We extract and purify high-quality DNA from a variety of sources, including genomic DNA, plasmids, and bacterial cultures.
Library preparation: We use advanced techniques to prepare highly complex libraries, including splitting DNA, ligating aptamers, amplifying with PCR, and size selection.
Specific cloning enzyme technology: We have extensive experience working with different types of cloning enzymes.
Quality Control: We perform strict quality control at every step of the process to ensure that our final libraries provide high quality results.
Methods
Restriction enzyme-based cloning: Restriction enzyme-based cloning involves cutting DNA into smaller fragments with restriction enzymes and then ligating aptamers for sequencing.
Gibson assembly: This is a one-step method that assembles multiple DNA fragments into a single sequence by using exonucleases, polymerases and ligases.
Golden Gate Assembly: Golden Gate Assembly is a modular method that uses an IIS-type restriction enzyme to produce sticky ends, allowing multiple DNA fragments to be ligated simultaneously.
Sample Requirements
Sample Quantity: We require a minimum of 500 ng of DNA for efficient library preparation.
Sample Quality: Starting material should be fresh and free of contaminants.
Sample Type: We can handle a variety of sample types, including genomic DNA, plasmids, and bacterial cultures.
Deliverables
High-quality sequencing libraries: Our state-of-the-art technology and experienced team ensure that we deliver high-quality sequencing libraries with high complexity and yield.
Customized reports: Upon request, we can provide custom reports detailing the specific protocols used to construct the libraries and any modifications made to meet your project requirements.
Our Features
Advanced technology: We use state-of-the-art technology and equipment to prepare highly complex libraries, including splitting DNA, ligating adapters, amplifying with PCR, and size selection.
Customization: We can customize our cloning enzyme library construction protocols to meet your specific needs, such as different sample types, downstream applications, and project requirements.
Affordable pricing: We offer competitive pricing for our cloning enzyme library construction services to ensure you get high quality sequencing libraries at an affordable cost.
Why Choose Us?
Creative Enzymes is a leading provider of cloning enzyme library construction services. Our state-of-the-art technology and experienced team ensure that we provide high-quality sequencing libraries to meet your specific needs. With our expertise, advanced technology, customization, quality control and affordable pricing, we are the ideal choice for your cloning enzyme library construction needs.
