Overview

DNA demethylation refers to the passage of DNA demethylation enzymes, including TDG (thymine DNA glycosylase), TET1 (methylcytosine dioxygenase 1) and TET2 (methylcytosine dioxygenase 2), which are essential from a range of studies of DNA demethylation, such as inhibitor screening, enzyme kinetics, selectivity analysis and protein arrays. DNA demethylation Methylation can be passive or active, and it is associated with epigenetic reprogramming of genes and important disease mechanisms. In some tumor tissues, 5-hmC levels are lower than in normal tissues and its deletion is considered as a possible biomarker for the diagnosis of cancer. Thus, DNA demethylation studies may lead to important discoveries with implications for cancer treatment.

Sepecific Assay Service We Offer

At Creative Enzymes, our DNA demethylase screening services are based on high quality and efficient chemiluminescence technology. In addition, we offer a large selection of DNA demethylases and substrates produced in-house to facilitate your epigenetic research.

• Creative Enzymes can screen for multiple inhibitors or targets through a set of assays.
• We can measure IC₅₀ (half maximum inhibitory concentration) and single point concentration..
• Creative Enzymes supports large scale high throughput screening services.
• Customers receive data within days of compound submission for efficient project guidance.

Methods

• Enzyme activity assays: These assays measure the catalytic activity of DNA demethylation enzymes in the presence of test compounds.
• Cell-based assays: Cell-based assays use cellular models to assess the effectiveness of the test compound in inhibiting DNA demethylase activity.

Sample requirements

We require a minimum of 1 μg of purified enzyme or cell lysate per assay. We recommend that samples be frozen at -80°C until shipment.

Our Features

• For each custom project, we assign a team of expert chemists and biologists to help our partners define their research path and goals.
• We also provide full assistance in realizing the scientific and business plan, as well as delivering the scientific and business plan.
• Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in DNA Demethylase inhibitor screening service. 

Deliverables

We provide our clients with detailed reports that include all experimental procedures and results obtained. The report includes a summary of the assay design, optimization, validation and data analysis.

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

At Creative Enzymes, our goal is to apply innovative technologies to DNA demethylase inhibitor screening service. We offer innovative, flexible and scalable solutions that can meet the needs of our customers. We strive to meet this challenge as a global company that places a high priority on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality. If you would like to know more about this service, please feel free to contact us.