There are two main methods commonly used by enzyme sequencing services: Edman degradation and mass spectrometry (MS) analysis. The most widely used method for protein sequencing and identification is mass spectrometry, and Edman degradation is one of the most important methods for characterizing the N-terminal end of proteins. Creative Enzymes provides mass spectrometry-based cloning enzyme sequencing analysis using nano LC-MS/MS nanoliter chromatography coupled with tandem mass spectrometry, as well as protein N-terminal sequence analysis services.

Custom Cloning Enzyme Sequencing Approaches We Offer

• Mass Spectrometry-based Protein Sequence Analysis

The advantages of mass spectrometry for enzyme sequence analysis over Edman degradation are that mass spectrometry is more sensitive, can cleave peptides faster, and can identify end-closed or modified proteins. creative Enzymes technology provides mass spectrometry-based protein sequence analysis services using existing high-resolution mass spectrometry technology platforms to achieve 100% coverage of the measured target protein sequences. coverage. This analysis can be used to confirm whether the recombinant protein is fully expressed, detect whether the recombinant protein expression process is broken, etc.

• Edman Degradation Method for Protein N-terminal Sequence Analysis

Edman degradation sequencing is a chemical method capable of obtaining amino acid sequence information at the N-terminal end of proteins. This method allows labeling and analysis of the N-terminal amino acid sequence without disturbing the peptide bond. The Edman degradation method has been widely used for N-terminal sequence analysis of protein samples. However, in practical applications, Edman degradation method has some limitations. Creative Enzymes 's Edman sequencing system to provide N-terminal sequencing services for purified protein products, protein vaccines and various protein samples for our researchers and research customers. With our sequencing system, up to 30 amino acids of N-terminal sequence information can be determined.

Service Preparation

There are many ways to perform sample preparation, and no particular method is the most effective way to prepare samples. The most common methods used by Creative Enzymes to prepare samples for protein sequencing include:

1. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
2. High performance liquid chromatography (HPLC)
3. Capillary electrophoresis (CE)

Service Process

Our Features

• Creative Enzymes works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
• Creative Enzymes has a proven, advanced cloning enzyme sequencing service technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.

Deliverables

• Results raw data
• Data analysis
• Results analysis
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a leading enzyme technology company offering a trusted suite of services and solutions, especially with our extensive experience in cloning enzyme sequencing service. Creative Enzymes can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.