Restriction enzymes are very important in the whole enzyme industry, and because of their unique functions in the field of bioengineering, they cannot be replaced by other enzymes. Therefore, it is of great importance to develop and apply them. Many restriction enzymes are produced by Gram-negative bacteria such as E. coli. These products are often contaminated with endotoxins, lipopolysaccharides (LPS) present in the cell walls of most Gram-negative bacteria. Creative Enzymes provides you with a variety of restriction enzyme endotoxin removal service to meet your scientific research needs.

Sepcific Restriction Enzyme Endotoxin Removal Service Approaches We Offer

Lipopolysaccharides (LPS) are the major components of the cell wall of Gram-negative bacteria. Endotoxin removal is necessary for a number of downstream applications, and Creative Enzymes' endotoxin removal service can reduce endotoxin levels to below 10 EU/mg during restriction enzyme production with a 98% success rate. We have successfully delivered several low endotoxin protein production projects that have met our customers' stringent endotoxin control requirements.

Service Contents

Our Techonology

• Molecular Exclusion Columns
• Ion Exchange Columns
• Protein Affinity Columns

Workflow

Creative Enzymes offers a one-stop solution for low endotoxin restriction enzyme production, including restriction expression and purification as well as endotoxin removal. Endotoxin control and removal begins with instrumentation and reagents, proceeds through various manipulations, and culminates in a variety of endotoxin removal technologies.

Figure 2. Workflow of our restriction enzyme endotoxin removal service.

Advantages

• Efficient endotoxin removal - guaranteed endotoxin levels below 10EU/mg
• Multiple strategies to solve endotoxin problems with 98% success rate
• One-stop shop from gene to low endotoxin restriction enzyme
• Predict loss rates at the beginning of the project to ensure adequate supply of low endotoxin restriction enzymes

Service Process

Our Features

• We have accumulated many years of essence in the field of enzyme service, helping customers to speed up project development and improve the overall success rate of the project.
• At Creative Enzymes, simply let us know your specific project needs. We will suggest the best strategy for you.

Deliverables

• Related results raw data
• Data analysis
• Image and result analysis
• Results analysis
• Activity assessment report
• Summary of relevant parameters

Why Choose Us?

Creative Enzymes is a professional enzyme service provider, supporting a variety of analysis services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. Creative Enzymes has accumulated years of quintessence in the field of restriction enzyme endotoxin removal service, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.